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.com
Volume 8, Issue 5 (Suppl)
J Blood Disord Transfus, an open access journal
ISSN:2155-9864
Hematology 2017
November 08-09, 2017
November 08-09, 2017 | Las Vegas, USA
11
th
International Conference on
Hematology & Hematological Oncology
Altered leucocyte functions in HIV infected subjects
Rosemary Omomo Ekpeh
and
Ajayi Olutayo Ifedayo
University of Benin, Nigeria
P
olymorphonuclear neutrophils play an important role inhost defense and theyhave the ability to recognize andphagocytose bacteria
and other microorganisms. Previous studies have shown that, leucocyte functions are impaired in human immunodeficiency
virus infection. This study was undertaken to investigate changes of phagocytic function and oxidative burst activity occurring in
HIV infected subjects. A total of 191 patients were recruited for this study, comprising 58 HIV negative individuals (control), 72
HIV infected subjects that are not on antiretroviral therapy and 61 HIV infected subjects on antiretroviral therapy. Trypan blue was
used to determine viability test, Nitroblue Tetrazolium test was used to measure the oxidative burst and phagocytosis was assessed by
incubating leucocyte suspension with
Escherichia coli
and measuring the ability of leucocytes to ingest bacteria. The CD4 cell count
and CD8 cell count was analyzed using BD FACSCount auto analyzer. Our results showed significantly decreased phagocytic function
and oxidative burst activity (p<0.05, respectively) in the HIV group both on ART and not on ART (untreated group) as compared
with the controls group. Similarly, a significant (p<0.05, respectively) decrease in leucocytes viability was observed in both HIV
groups compared with controls. Furthermore, leucocyte viability of HIV infected subjects who were not on ART were significantly
reduced (P<0.05) when compared with HIV infected subjects on ART. This finding may suggest that leucocytes from HIV infected
individuals have impaired ability to phagocytose and undergo oxidative burst activity, however may contribute to the increased risk of
bacterial infections in HIV-infected subjects. It was observed that oxidative activity and phagocytic function was inversely correlated
to the change in CD4 count value, that is, the greater the CD4 value the better the oxidative activity and phagocytic function. It is
recommended that further studies on mechanisms of failure of phagocytosis and oxidative burst potentials of HIV infected subjects.
Biography
Rosemary Omomo Ekpeh is a graduate of Medical Laboratory Science with a bias in Hematology and Blood Transfusion Science. She is currently pursuing MSc in Hema-
tology at the University of Benin, Nigeria. She works as a Medical Scientist with the APIN/PEPFAR Laboratory of Edo State Health Management Board.
rm1202002@yahoo.co.ukRosemary Omomo Ekpeh et al., J Blood Disord Transfus 2017, 8:5 (Suppl)
DOI: 10.4172/2155-9864-C1-028