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Volume 09 Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X Gastro Congress 2019
July 08-09, 2019
July 08-09, 2019 | Zurich, Switzerland
14th Euro-Global Gastroenterology Conference
Nitric oxide donor, S-nitrosoglutathione, to maintain intestinal barrier integrity: Potential therapeutic
candidate for prevention of inflammation recurrences
Romain Schmitt1, Haiyan Yu1, Pierre Leroy1, Franck Hansmannel1,2, Igor Clarot1, Isabelle Lartaud1 and Anne Sapin-minet1
1University of Lorraine, France
2NGERE Inserm U1256 Lab, France
Nitric oxide (NO) is known to play a pivotal role to maintain the intestinal barrier integrity, such as regulation
of oxidative stress, healing, mucus secretion, immune system regulation, etc. S-nitrosoglutathione (GSNO), a
nitric oxide donor is naturally secreted by enteric glial cells after stimulation of the vagus nerve. GSNO is known
to prevent inflammatory events and to preserve intestinal barrier integrity. We have highlight in a Ussing chamber
model that there is a concentration-dependant effect of NO on rat ileon intestinal permeability: A low concentration
of GSNO (0.1 µm) significantly decreases the permeability of sodium fluorescein after two hours when compared to
high concentrations (100 µm). This effect is not observed in the presence of glutathion equivalent concentrations.
Moreover, GSNO degradation and absorption on isolated rat intestine were studied and we found that an enzymatic
activity of gamma-glutamyl-transpeptidase expressed on intestinal epithelioma (and also by microbiota), is involved
in GSNO intestinal permeability. Also, the inhibition of endogenous secretion of NO by using N-nitro-L-arginine
methyl ester (NO synthases inhibitor) showed us that NO observed effect in intestinal permeability comes from
exogenous supply with GSNO. From these results, GSNO could be proposed as an innovative prophylactic agent, in
order to prevent relapses of inflammation for inflammatory bowel diseases patient in clinical remission.
Recent Publications
1. Cheadle G A, et al., (2013) Enteric glia cells attenuate cytomix-induced intestinal epithelial barrier breakdown.
PloS one, 8(7):e69042.
2. Savidge T C, et al., (2007) Enteric glia regulate intestinal barrier function and inflammation via release of
S-nitrosoglutathione. Gastroenterology 132(4):1344-1358.
3. Hanigan M H and Frierson H F (1996) Immunohistochemical detection of gamma-glutamyl transpeptidase in
normal human tissue. J Histochem Cytochem 44(10):1101-1108.
Biography
Romain Schmitt is pursuing his PhD and is currently working at the NGERE Inserm U1256 Lab, France. He has already published few papers with his colleagues
and is currently working on many papers that will be published in the upcoming year.
Romain Schmitt et al., J Gastrointest Dig Syst 2019, Volume 09