gastro-congress-2016_posters-accepted-abstracts

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Volume 6, Issue 7(Suppl) J Gastrointest Dig Syst 2016
ISSN: 2161-069X JGDS, an open access journal
Page 70
Gastro Congress 2016
October 24-25, 2016
conferenceseries.com
October 24-25, 2016 Valencia, Spain
9th Euro Global
Gastroenterology Conference
J Gastrointest Dig Syst 2016, 6:7(Suppl)
Laparoscopic uncut Roux-en-Y anastomosis in the distant gastrectomy for gastric cancer: A
report of 92 patients
Ze-Kuan Xu, Li Yang, Hao Xu, Diancai Zhang and Fengyuan Li Linjun Wang
The First Affiliated Hospital of Nanjing Medical University, China
Objective: The aim of this study was to investigate the safety and feasibility of laparoscopic uncut Roux-en-Y anastomosis in
the distant gastrectomy for gastric cancer.
Methods: The clinical data of 92 patients performed laparoscopic uncut Roux-en-Y anastomosis in the Department of Gastric
Surgery, the First Affiliated Hospital of Nanjing Medical University from June 2014 to June 2016 were analyzed retrospectively.
Results: The operations were performed successfully for all patients. The mean operation duration, anastomosis time, blood
loss and the number of lymph nodes dissection during the surgery were (178.67±29.12) min, (30.84±7.41) min, (48.78±35.64)
ml and 34.43±9.84 respectively. The time spent before gastrointestinal motility, liquid-diet intake, out-of-bed ambulation
and the average hospitalization days after operation were (76.40±21.47) hours, (5.30±1.25) days, (48.14±20.25) hours and
(9.19±3.09) days respectively. Postoperatively, 6 patients experienced complications, including gastrointestinal bleeding (2
patients), duodenal stump fistula (1 patient), Chylorrhea (1 patient), Roux-Y stasis syndrome after gastrectomy (2 patients), all
of the complications were cured conservatively. None of the other complications occurred, such as anastomotic fistula, anasto-
motic stenosis, infection of incision, etc.
Conclusion: The laparoscopic uncut Roux-en-Y anastomosis is safe and feasible in the distant gastrectomy with D2 dissection
for gastric cancer, with advantages of less trauma, faster recovery and so on.
Standardization of an isolation protocol of group a rotaviruses among neonatal diarrheic calves,
Morocco
Ennima Imane1, 2, Sebbar Ghizlane2, Harif Bachir2, Amzazi Saaid1, Loutfi Chafiqa2 and Touil Nadia2
1University Mohammed V, Morocco
2Société de Productions des Produits Biologiques and Vétérinaires, Morocco
Group A rotaviruses (RVA) are the main cause of neonatal calve diarrhea (NCD) in Morocco. Until now, no reports are
available regarding isolation and cultivation of RVA in clinical samples fromMoroccan domestic animals or children with
acute gastroenteritis (AGE) and burden due to this infection in animals remain unknown. Hence, this study aims to isolate
RVA strains from NCD clinical samples in order to support RVA disease control in Morocco. This isolation process constitutes
a first step toward vaccine development. Thirteen fecal samples were obtained from calves with a single episode of neonate
calf diarrhea at three different dairies and two samples were collected from field during a severe NCD outbreak. Diagnosis of
RVA infection was based on fecal immune-chromatographic rapid test and further evaluated for their hemagglutination (HA)
activity. RVA isolation was carried out on MA104 cells after inoculates were treated with different concentrations of trypsin
TPCK. All RVA isolates were confirmed by LSI VetMAX™ Triplex Ruminant Rotavirus and Coronavirus Real-Time PCR kit.
RVA isolation was achieved for nine clinical samples following one or two passages (60%) and was properly depended on HA
activity and trypsin treatment of inoculates. The first sign of CPE detected consisted of increased cell granularity, obscure
cell boundaries, cell rounding, and eventual degeneration and detachment of cells. These results constitute a crucial and a
necessary step allowing preventive and veterinary medicine to support RVA disease controls in the country.