gastro-congress-2016_posters-accepted-abstracts

Volume 6, Issue 7(Suppl)

J Gastrointest Dig Syst 2016

ISSN: 2161-069X JGDS, an open access journal

Page 62

Gastro Congress 2016

October 24-25, 2016

conferenceseries

.com

October 24-25, 2016 Valencia, Spain

Euro Global

Gastroenterology Conference

Activation of Macrophage and Its Impact on structural defects of rat Liver in experimental

Diabetes

Danilova I, Gette I, Abidov M.

Ural Federal University, Russian Federation

Statement of the Problem

: The Morphofunctional state of the liver in diabetes mellitus should be assessed since all metabolic

processes occur in liver; hepatocytes have the insulin-dependent mechanism of glucose metabolism and 40-60% of insulin

is inactivated in liver. The structure of damaged liver tissue and mechanisms of hyperglycemia which cause the damage were

described in many investigations. The assessment of various methods of damaged liver correction was performed. It is known

that hepatic macrophages play key role in tissue injury and repair. However, its effects on regenerative processes in diabetic

liver are not investigated enough. We previously set that the macrophage modulator 3-aminophthalhydrazid (3-APH) activated

anti-inflammatory and antioxidant functions in monocyte-macrophage cells.

The purpose of this study

: is to evaluate the impact of macrophages activated by 3-APH on structural defects in diabetic rat

liver.

Methodology

: A damaged liver tissue and hepatocyte reparation were studied in alloxan diabetic rats after modulation of

macrophage activity by 3-APH during 30 and 60 days.

Finding

: The morphological study of diabetic liver tissue revealed degenerative changes in hepatocytes, inflammatory

processes in the portal and intralobular stroma, microangiopathy and blood circulation disorders. Structural disorders of liver

parenchyma increased at a prolongation of AD up to 60 days. It was found that the administration of 3-APH contributed to

reduction of damage and restoration of normal liver histological structure due to activation of reparative processes.

Conclusion

: Thus, macrophage activation in diabetic liver causes increase in hepatocyte activity and compensation of

structural damage in liver tissue. 3-APH is likely to be a promising drug for correction of liver tissue in diabetes due to its

hepato protective effect.

The study is supported by the grant from the Russian Science Foundation, the project № 16-15-00039.

Figure 1

: a - histological structure of intact rat liver tissue. b –diabetic liver tissue (30 days): 1- plethora in central venules, 2-

lymphocytic infiltration of sinusoidal capillary, 3 - degeneration of hepatocytes. c – diabetic liver tissue (60 days): 1 - intralobular

necrosis of hepatocytes, 2 - edema, vasodilatation, 3 - desquamation of endothelial of sinusoidal capillaries. d –diabetic liver

tissue (60 days) treated by 3-APH: 1 - polyploid hepatocytes, 2 – dual-nucleus hepatocytes. Staining by hematoxylin-eosin.

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Danilova I, J Gastrointest Dig Syst 2016, 6:7(Suppl)