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Volume 6, Issue 6(Suppl)
J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 83
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries
.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7
th
Euro-Global Summit on
mRNA and miRNA expression patterns associated to pathways linked to metal mixture health
effects
Rojas E, Martinez Pacheco M, Rodriguez Sastre A
and
Valverde M
Universidad Nacional Autonoma de Mexico, Mexico
M
etals are a major category of globally distributed pollutants that tend to accumulate in select tissues. Metal mixtures are
a potential threat to human health by increasing disease risk. Recently, experimental data have linked altered miRNA
expression with exposure to several metals, including As, Cd and Pb. Although, several human populations are exposed to
low concentrations of As-Cd-Pb mixture, there are few data at respect to miRNA expression patterns. Thus, this study aims to
evaluate global miRNA and mRNA expression changes induced by a metal mixture (NaAsO
2
, CdCl
2
and Pb(C
2
H
3
O
2
)
2
•3H
2
O)
to explore the role of miRNA-222 as a post-transcriptional regulator of
RAD51C
, a gene involved in homologous recombination
of double-strand break DNA repair. Our results show that miRNA expression profile responsible for the mRNA expression
changes induced by metal mixture exposure are involved in cellular processes, including DNA repair, cell death, growth and
proliferation related to the metal-associated pathologies (cardiovascular diseases and cancer). On the other hand, we found
that miR-222 directly negatively regulates
RAD51C
expression and impairs homologous recombination of double-strand break
DNA repair, generating genetic instability that could be related with cell transformation.
Biography
Rojas E has completed his PhD from UNAM, Mexico. He is a Full Professor in Biomedical Research Institute. He has published more than 75 papers in reputed
journals that has been cited more than 4500 times, and had been serving as an Editorial Board Member of
Mutation Research Reviews
and
Mutation Research
Genetic Toxicology and Environmental Mutagenesis.
emilior@biomedicas.unam.mxRojas E et al., J Clin Toxicol 2016, 6:6(Suppl)
http://dx.doi.org/10.4172/2161-0495.C1.021