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Volume 6, Issue 6(Suppl)
J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 79
Euro Toxicology 2016
October 24-26, 2016
conferenceseries
.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7
th
Euro-Global Summit on
Mechanistic study of TEMPO-associated oxidative stress and genotoxicity
Nan Mei, Xiaoqing Guo, Zuhong Zhang, Stacey L Dial, Vasily N Dobrovolsky, Si Chen
and
Lei Guo
National Center for Toxicological Research, USA
T
hebiologicalconsequencesofexposuretopiperidinenitroxidesisaconcern,giventheirwidespreaduseinmanufacturingprocesses
and their potential use in clinical applications. Previously, we have demonstrated that TEMPO (2,2,6,6-tetramethylpiperidine-
1-oxyl), a low molecular weight free radical, can induce cytotoxicity and genotoxicity in mammalian cells. Extending this earlier
work, the present study investigates the underlying mechanisms of TEMPO-associated oxidative stress and genotoxicity, particularly
the roles of reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) signaling. Our results demonstrate that
TEMPO induced time- and concentration-dependent intracellular ROS production and glutathione depletion in mouse lymphoma
L5178Y cells. TEMPO also induced apoptosis as demonstrated by increased caspase-3/7 activity, an increased proportion of annexin
V stained cells, and decreased expression of anti-apoptotic proteins including Bcl-2, Bcl-xL and Mcl-1. N-acetylcysteine, a ROS
scavenger, attenuated the ROS production and apoptosis induced by TEMPO. Moreover, Western blot analyses revealed that
TEMPO activated γ-H2A.X, a hallmark of DNA damage, and c-Jun N-terminal kinases (JNK); a key member in the mitogen-
activated protein kinase (MAPK) signaling pathway. Addition of SP600125, a JNK-specific inhibitor, blocked TEMPO-mediated
JNK phosphorylation and also attenuated TEMPO-induced apoptosis. These findings indicate that TEMPO-induced apoptosis and
toxicity are, at least in part, mediated by oxidative stress and activation of JNK in the MAPK pathway.
Biography
Nan Mei after graduating from Hebei Medical University, China in 1984, started his scientific career in clinical cancer research and diagnosis as a Physician in a
university hospital. In 1997, he received his PhD degree from the University of Occupational and Environmental Health, Japan. He then extended his training on
assessing DNA damage as a Post-doctoral Fellow at the Cross Cancer Institute, Canada. In 2002, he joined the US FDA/NCTR. Currently his research focuses on
genetic toxicology and toxicogenomics. He has published more than 80 peer-reviewed research articles in prestigious journals and 11 book chapters.
Nan.Mei@fda.hhs.govNan Mei et al., J Clin Toxicol 2016, 6:6(Suppl)
http://dx.doi.org/10.4172/2161-0495.C1.021