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Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 71
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
Potentialities of synchrotron radiation (SR) fourier transform infrared microscopy (FTIRM) for
environmental toxicology and pharmacology
Lisa Vaccari
Elettra Sincrotrone Trieste, Italy
The setting up of new methodologies for the assessment of material toxicology is a field of research continuously evolving in
order to answer the new and urgent questions of the modern era. In this framework, SR-FTIRM is emerging as a valuable
tool for in vitro and ex vivo toxicological studies. The technique is able to provide biochemical information on the sample
under investigation in a label-free, safe and spatially-resolved manner, through the investigation of the vibrational motions of
the molecular constituents. In this presentation, a short introduction of Elettra Sincrotrone Trieste, III generation Synchrotron
Facility in Italy will be given, focusing on the activities in the toxicology field at SISSI beamline, the infrared laboratory at
Elettra. Several topics will be covered, such as: The chemical characterization of asbestos bodies versus environmental
particulates (anthracosis) in human lung tissues from asbestos exposed and control patients; the biochemical modifications
on crustacean (Porcellio scaber) digestive glands upon exposure to diverse nanoparticles, (tungsten, zinc and silver oxides), the
concentration of which in environmental systems is increasing as a consequence of anthropogenic activities. The correlation
between chemical state, concentration and shape of the nanoparticles on animal toxicity will be highlighted and; an overview
on the in vitro capabilities of SR-FTIRM will be given, focusing on the possibility to monitor in real-time on live single cells
the effects of therapeutics and stressor agents in general. A comparison with more conventional analytical approaches such as
flow cytometry will be presented.
Biography
Lisa Vaccari has been the Leader of the SISSI infrared beamline at the Elettra Sincrotrone Trieste since 2006. She is an expert in Bio-spectroscopy and pioneered
the exploitations of microfabrication capabilities for the design and fabrication of IR-suitable microfluidic devices for performing in vitro bio-experiments under
physiological conditions. She has experience in several other analytical techniques and she is actively involved in several projects in the fields of “Environmental
toxicology, toxicology of nanomaterials, cellular toxicology of drugs and chemicals”.
Lisa Vaccari, J Clin Toxicol 2016, 6:6(Suppl)