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conferenceseries
.com
Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
Expression of major virulens genes of Listeria monocytogenes isolated from cattle, sheep and chicken
Naim D Ayaz
1
, Gizem Cufaoglu
1
, Bahar Onaran
2
, Muammer Goncuoglu
2
, Fatma Seda Ormanci
2
and Irfan Erol
3
1
Kirikkale University, TURKEY
2
Ankara University, TURKEY
3
Ministry of Food, Agriculture and Livestock, TURKEY
S
tatement of the Problem: Listeria monoctogenes is a zoonotic food-borne bacteria that leads to a variety serious infections in
humans such as encephalitis, meningitis, abortion and septicemia. Most L. monocytogenes strains can cause high morbidity and
mortality depending on their virulence, however some strains don’t cause any infections in mammals due to absence of their virulence
factors. L. monocytogenes provides its pathogenicity with virulence factors such as hlyA, actA, inlA, inlB, inlC, inlJ, plcA, plcB genes
and vip, fbpA and fri which are recently reported. The presence of these genes is just as important as the expression levels that play
an important role on its pathogenicity. Therefore, this study was aimed to detect the important virulence genes and expression levels
of L. monocytogenes isolated from cattle, sheep carcasses and chicken (broiler) neck skin samples during slaughtering in Turkey.
Methodology & Theoretical Orientation: In the study 31 L. monocytogenes isolated from 5 cattle, 3 sheep carcasses and 10 chicken
neck skin samples were analyzed by real time RT-PCR for the presence and expression levels of major virulence genes including hlyA,
actA, inlA, inlB, inlC, inlJ, plcA, plcB, vip, fbpA and fri. In the study spoG was used as house-keeping gene. Findings: According to
the real time RT-PCR, hlyA, actA, inlA, inlB, inlC, inlJ, plcA, plcB, fbpA and fri genes were detected from all the isolates. However
5 isolate were not harbor vip gene. Six virulence genes were up regulated in a chicken isolate that has the highest virulence potential
compared with the other L. monocytogenes isolates. Conclusion & Significance: Most of the L. monocytogenes isolates harbored
all the 11 virulence genes. Some were up regulated, some were down and some were expressed as same as the house-keeping gene.
Genetically, most virulent L. monocytogenes was originated from chicken and its serotype was 1/2a.
Biography
Naim Deniz Ayaz is Professor of the Department of Food Hygiene and Technology at Kirikkale University Faculty of Veterinary Medicine. He received his PhD
in Food Hygiene and Technology from Ankara University in 2008. His main research interests are food microbiology, characterization of food-borne pathogens,
bacteriophages, biocontrol of pathogens and bacterial antibiotic resistance.
naimdenizayaz@kku.edu.trNaim Deniz Ayaz, J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-032