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Volume 5, Issue 6(Suppl) J Infect Dis Ther, an open access journal
ISSN: 2332-0877 Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6th Euro-Global Conference on
Molecular microbiology as a modern platform for rapid, specific, sensitive and unlimited detection of pathogenic
microorganisms
Tereza Jancuskova
KitGen , czech republic
Molecular microbiology is a novel concept that opens fascinating possibilities in the pathogen detection. Many microorganisms
are fastidious or uncultivatable; their cultivation time is unacceptably long; are of high epidemiological importance; or require
sophisticated cultivation conditions. Molecular techniques allow for quantitative detection of microorganisms based solely on the
presence of their unique DNA or RNA sequences. Molecular microbiology enables to identify causative infectious agents even in
those situations when standard cultivation-based microbiology fails. Since 2006, we have developed over 500 single and multiplex
quantitative Real-Time PCR assays to detect pathogenic and opportunistic infectious agents relevant for both human and veterinary
clinical settings. We have implemented pandetection approach to detect bacteria and fungi based on Sanger sequencing. For the
most challenging biological samples (gut microbiome) we have also developed a pandetection strategy based on Next Generation
Sequencing (NGS). Using this combined approach, we can identify microbial agents with the widest detection range possible
(pandetection), quantify the load of individual microorganisms in the sample and provide the clinician with the result within hours
(Real-Time PCR), or 2-3 days maximum (Sanger sequencing or NGS). Over the past 10 years, we have diagnosed more than 30,000
biological samples, originating from both human and veterinary patients. They covered hyperacute clinical settings (sepsis), chronic
and underdiagnosed diseases, and emerging zoonoses (our finding of a novel zoonotic agent Candidatus, Neoehrlichia Mikurensis
transmissible by a tick bite, with unexpected Central and Western European geographic occurrence).
Biography
Tereza Jancuskova has completed her Graduation from Charles University in Prague, Czech Republic in 2008. She has continued her PhD studies at the Third
Medical Faculty, Charles University in Prague, specializing in Genetics, Molecular Biology and Virology. She has received her PhD degree in 2015. She has
extensive expertise in Molecular Haemato-Oncology and Molecular Genetics, both in human and veterinary medicine.
Tereza Jancuskova, J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-032