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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 24
Standardization of products of biotechnological sericulture by parthenocloning and cryobanking
of transgenic clonal silkworms
Valeriya Zabelina, Naoyuki Yonemura, Keiro Uchino, Hideki Sezutsu, Vyacheslav Klymenko, František Sehnal, Yuji Mochida, Yoko Takemura
and
Toshiki Tamura
National Agriculture and Food Research Organization, Japan
C
urrentgeneticengineeringofthesilkwormenablestoproducenewkindsofsilkandtousesilkwormsasproteosynthetic
bioreactors for obtaining precious proteins. Nowadays a lot of target products derived from the silkworm are already
used in many areas of life from cosmetics through pharmacy, regenerative medicine to cosmonautics. The problem of
product standardization we propose to solve by easy and inexpensive decision – parthenocloning, which enables rapid
fixation of transformed genotypes and conservation of any unique man-made complexes. Parthenocloning is based on
exact copying of maternal genome and a single female initiates a clonal line with the same genetic and morphological
traits, which can be easily maintained without sexual reproduction as exclusively female populations. Such populations
exist already for more than 50 years as genetically stable females whose unfertilized eggs are induced to develop by
heat-shock treatment. This is the best time-tested evidence of genome stability. We constructed new non-diapausing
parthenogenetic strains and developed efficient injection method adapted for the eggs of parthenoclone. We showed
that transgenic silkworms could be obtained in high frequency and propagated as clonal populations. We obtained
successful transgenesis in a parthenoclone and inserted transgenes were faithfully transferred to successive generations.
We showed the possibility of cryopreservation of ovaries of transgenic clonal strains and obtained recovery as individuals
by ovary transplantation into female larvae, which opens door for cryobanking of any specific standardized genotypes
used for obtaining of precious target products.
Biography
Valeriya Zabelina has completed her PhD in the field of Genetics at the age of 26 years from Karazin Kharkiv National University, Ukraine, was involved
in teaching of Developmental biology and worked at the Laboratory of Germ and Stem Cells at the same University, Biology Faculty, Chair of Genetics
and Cytology. Then continued her postdoctoral studies at the Biology Centre of the Czech Academy of Sciences, Czech Republic under several European
projects (Mobitag, Postdoc Bioglobe), containing research stays abroad (Spain, Japan) and presenting her results at international meetings. She was also
involved in teaching of Developmental biology and supervision of a student at the University of South Bohemia, Czech Republic. At the moment she is
conducting her research in Japan under JSPS fellowship with the group working in transgenic silkworm. She has been a member of Ukrainian Society of
Genetisists and Breeders and Japanese Sericultural Society.
zabelina.valeriya@gmail.comValeriya Zabelina et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-100