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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21st European
Biotechnology Congress
conferenceseries.com
Page 36
BACTEC MGIT 960 system and classic Lowenstein-Jensen culture in the diagnosis and drug
susceptibility of Mycobacterium tuberculosis from pulmonary specimens, at the Pasteur Institute
of Algeria
Ferhat Djoudi1, Dalila Benremila1, Rym Touati2, Sabrina Kassa 1, Massilva Messaoudi1, Randa Yahi2 and Djamal Yala2
1Abderrahmane Mira University of Bejaia, Algeria
2Pasteur Institute of Algeria, Algeria
Tuberculosis is an old infectious disease and the causative agent is Mycobacterium tuberculosis complex. The direct
diagnosis stills long and fastidious since bacilloscopy, even if is fast, lacks sensitivity. The culture on Lowenstein-
Jensen (L-J), which remains the reference method with a good sensitivity, sometimes takes up to ten weeks to obtain the
result. In order to compensate the slow growth of cultures on solid media, new automated methods have been developed,
including BACTECMGIT 960, Versa TREK, MBRedox, BACTEC 460, which allow early diagnosis and more suitable for
antibiotic therapy, in addition to their good sensitivity and specificity. The aim of this study is to verify the contribution
of BACTEC MGIT 960 in the diagnosis of pulmonary tuberculosis, compared to basciloscopy and classic culture on
L-J medium, at the Tuberculosis and Mycobacteria unit in Pasteur Institute of Algeria. Nine hundred and fourteen
(914) specimens were collected between January 2016 and April 2017. One hundred and seventy nine (179) cases were
reported positive by L-J classical culture and/or BACTEC MGIT 960. Among the 179 cases, 155 were detected by the
BACTEC MGIT 960 system, and confirmed by Ziehl control, L-J subculture and MPT64 immuno-chromatographic
assay. On classic L-J culture and bacilloscopy, nevertheless, only 123 and 95 specimens respectively were positive. These
results confirm the height susceptibility of BACTECMGIT 960 in improving the diagnosis of tuberculosis in bacilli-poor
specimens, compared to classic culture (p=0.037) and direct examination (p=0.014). Furthermore, the contamination
rate was higher in L-J culture: 81/914 (8.86%), including 7 bacilloscopy positive specimens, whereas, with BACTEC
MGIT 960, only 29/914 (3.17%) specimens were contaminated, with no positive bacilloscopy cases. This result was
statistically confirmed (p<0.0001). However, on the 95 bacilloscopy positive specimens, 6 did not give positive cultures
neither on BACTEC MGIT 960 nor on L-J. The main advantage of BACTEC MGIT 960 is its ability to shorten the time
of mycobacterial growth to an average of 7 days, compared to the solid medium. Nevertheless, the bacilloscopy and
culture on L-J remains complementary to this automat, for a reliable diagnosis. Despite the good laboratory practices,
there is an incompressible risk of contamination.
Biography
Ferhat Djoudi has completed his PhD on Epidemiology and molecular characterization of MRSA in 2015 at Abderrahmane Mira University of Bejaia,
Algeria. And he started Postdoctoral studies at the same university, on MDR and XDR tuberculosis in Algeria. He is the Head of Microbiology
Department and Teacher-Researcher at the same university. He has published many papers in reputed journals.
Ferhat Djoudi et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-100