euro-biotechnology-2018-scientifictracks

Notes:

Volume 8

Journal of Biotechnology & Biomaterials

ISSN: 2155-952X

Euro Biotechnology 2018

October 11-12, 2018

October 11-12, 2018 | Moscow, Russia

European

Biotechnology Congress

conferenceseries

.com

Page 34

Elevated expression of cytosolic phospholipaseA

delta is associatedwithhepatocellular carcinoma

progression: Animal study validated with sera of liver cancer patients

Maryam Ranjpour Aghmiouni

and

S K Jain

Jamia Hamdard University, India

iver cancer is the third common cancer to cause maximum death among patients diagnosed with cancers. The search

for new biomarker discovery is necessary as none of the identified biomarkers alone are enough sensitive toward

hepatocellular carcinoma (HCC). In order to find out novel biomarkers that can diagnose HCC at very early stage, we

have developed a rodent model using chemical carcinogens, N-Nitrosodiethylamine (DEN) and 2-aminoacetylfluorine

(2AAF). The disease progression was monitored by histological evaluation. Proteomic approaches such as

2D-Electrophoresis, LCMS/MS andWestern blot analyses have been used to analyze the differentially expressed proteins

in carcinogen-treated animals vis-a-vis controls. The total serum proteins were isolated, solubilized and resolved on

2D-Gel Electrophoresis using broad pH range IPG strips. PD-Quest analysis revealed proteins that are differentially

expressed in serum of the carcinogen-treated rats as compared to controls. Some of these proteins have been identified

by LCMS/MS. Histological analysis confirmed liver inflammation and disease initiation at one month and tumorigenesis

at four months after carcinogen treatment. One of the differentially expressed proteins, namely, cytosolic phospholipase

delta was significantly up-regulated at very early stage of cancer development and continued to remain elevated

with disease progression up to tumor stage. The increase in its expression has been confirmed by Western blot analysis.

Further, the analysis of serum of liver cancer patients also showed elevated expression of this protein that validated our

experimental data. The study suggests that elevation in cytosolic phospholipase A

delta expression is associated with

progression of HCC.

Biography

Maryam Ranjpour Aghmiouni has completed her PhD from Jamia Hamdard University. She has applied for Post-doctoral position at Illinois University

and her application is under process. She has published two manuscripts and three more manuscripts are under re-revision status at high repute

journals.

Maryam Ranjpour Aghmiouni et al., J Biotechnol Biomater 2018, Volume 8

DOI: 10.4172/2155-952X-C5-100