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Volume 8, Issue 2 (Suppl)
J Biosens Bioelectron, an open access journal
ISSN: 2155-6210
Euro Biosensors 2017
July 10-11, 2017
July 10-11, 2017 Berlin, Germany
7
th
Euro Biosensors
and Bioelectronics Conference
Detection of gold nanoparticles aggregation growth induced by nucleic acid through laser scanning
confocal microscopy
Ramla Gary
University of Calabria, Italy
T
he gold nanoparticle (GNP) aggregation growth induced by deoxyribonucleic acid (DNA) is studied by laser scanning confocal
and environmental scanning electron microscope. As in the investigated case, the direct light scattering analysis is not suitable,
we observe the behavior of the fluorescence produced by a dye and we detect the aggregation by the shift and the broadening of
the fluorescence peak. Results of laser scanning confocal microscopy images and the fluorescence emission spectra from lambda
scan mode suggest, in fact, that the intruding of the hydrophobic moiety of the probe within the cationic surfactants bilayer film
coating GNPs results in a Förster resonance energy transfer. The environmental scanning electron microscopy images show that DNA
molecules act as template to assemble GNPs into three-dimensional structures which are reminiscent of the DNA helix. This study is
useful to design better nano-biotechnological devices using GNPs and DNA.
Biography
Ramla Gary has completed her PhD in 2017 from the Laboratory of Liquid Crystals and Interfaces in Physics department in collaboration with the Biological
department at University of Calabria, Italy, and Post-doctoral studies from the same university. She has published more than five papers in reputed journals, and
has participated in more than eight international and national conferences.
gary-ramla@hotmail.comRamla Gary, J Biosens Bioelectron 2017, 8:2(Suppl)
DOI: 10.4172/2155-6210-C1-033