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Volume 8, Issue 2 (Suppl) J Biosens Bioelectron, an open access journal
ISSN: 2155-6210 Euro Biosensors 2017
July 10-11, 2017
July 10-11, 2017 Berlin, Germany
7th Euro Biosensors
and Bioelectronics Conference
Secure accuracy at increased precision of AFM-probe integrated biosensor
Sarmiza Elena Stanca, Matthias Urban, Frank Häntschke, Gabriel Zieger and Wolfgang Fritzsche
Leibniz Institute of Photonic Technology, Germany
The plasma membrane regulates the selective interchange of matter between the interior and the exterior of the cell. Understanding
this complex process requires knowledge of the plasma membrane´s molecular constituents. Topical reports prove the access to
the molecular level of the synthetic membrane by atomic force microscopy (AFM). This technique also permits an electrochemical
investigation in the immediate vicinity of the tip. An electrochemical and topographic study of the living cell membrane, by the mean
of an AFM-probe integrated amperometric biosensor is employed to localize specific molecules in the natural cellular membrane
(Figure 1). Several materials and shapes of the AFM probes integrated in different systems are presented. It is underlined that the
selection of control experiment is decisive in achieving accurate findings. The central concern of this study is how to preserve the
sensor response accuracy while increasing its precision.
(a)
(b)
(c)
Figure 1: (A) Height, amplitude and phase atomic force micrographs (110 µm x 110 µm) of the cells immobilized on conductive glass;
(B) Height, amplitude and phase AFMs in one location of 1.5 µm x 1.5 µm of the plasma membrane; (C) AFM probe integrated sensor
signal on two different points: green and red marked on the AFM image (200 nm x 200 nm).
Biography
Sarmiza Elena Stanca has her expertise in electrochemical and optical nanosensors achieved during her research activity at the EPFL Lausanne (Swiss
Confederation Fellow), UCD Dublin (Marie-Curie-Fellow), UKJ Jena (Marie-Curie-Fellow), University Babes-Bolyai Cluj-Napoca, Research Centre Karlsruhe and
IPHT Jena (DAAD Fellow). She is currently a Scientist at the Leibniz Institute of Photonic Technology, Jena.
Sarmiza Elena Stanca et al., J Biosens Bioelectron 2017, 8:2(Suppl)
DOI: 10.4172/2155-6210-C1-033