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Volume 09
Journal of Alzheimers Disease & Parkinsonism
ISSN: 2161-0460
Epilepsy 2019
Parkinsons Congress 2019
August 29-31, 2019
JOINT EVENT
conferenceseries
.com
August 29-31, 2019 Vienna, Austria
&
5
th
International Conference on
Epilepsy & Treatment
5
th
World Congress on
Parkinsons & Huntington Disease
Derivation of dopaminergic neurons from embryonic stem cells using a silk nanofibrous scaffold
Maryam Nazm Bojnordi
1
, Ebrahimi-Barough S
2
, Vojoudi E
2
and
Ghasemi H
1
1
Mazandaran University of Medical Sciences, Iran
2
Tehran University of Medical Sciences, Iran
T
he limited capcacity of the central nervous system in repairmen of neuronal population such as dopaminergic
neuron cells suggests stem cell therapy for neurogenesis in Parkinson’s disease. Also, stem cell therapy
accompanied with scaffolds, is a promising treatment in neural tissue engineering to induce neural differentiation
in damaged tissue of brain. Here we fabricated and used a silk nano fibrous scaffold for differentiation of embryonic
stem like cells in to dopaminergic neuron cells. Embryonic stem cells, were cultured on fabricated Silk scaffolds. The
neural differentiation was induced using a modified technique includes; culturing in the presence of Retinoic acid
and neurobasal medium with 10 ng/ml epidermal growth factor, 20 ng/ml basic fibroblastic growth factor for 10
days. The neural differentiation was investigated using the evaluation of specific markers via immunocytochemistry
and real-time technique. Our dates proved that silk scaffold support the differentiation of embryonic stem cells cells
in to dopaminergic neuron. The expression of neural specific markers were significantly higher in the cells were
cultured on fabricated Silk scaffolds in compare to monolayer control group. Electrospun silk nano fibrous scaffold is
considered as a biological substitutes for neural differentiation of stem cells that is a crucial step in tissue engineering
for neural tissue repair and regeneration.
Biography
Maryam Nazm Bojnordi has completed his PhD at Tarbiat Modares University. She is a Member of Scientific Staff of Anatomical Science at Mazandaran
University of Medical Sciences. She has published more than 32 papers and has been serving as an Editorial Board Member of journals.
Maryam Nazm Bojnordi et al., J Alzheimers Dis Parkinsonism 2019, Volume 09