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Volume 09
Journal of Alzheimers Disease & Parkinsonism
ISSN: 2161-0460
Epilepsy 2019
Parkinsons Congress 2019
August 29-31, 2019
JOINT EVENT
conferenceseries
.com
August 29-31, 2019 Vienna, Austria
&
5
th
International Conference on
Epilepsy & Treatment
5
th
World Congress on
Parkinsons & Huntington Disease
Transdifferentiation of human umbilical cord-derived mesenchymal stem cells to dopaminergic neurons
in three-dimensional culture
Hatef Ghasemi Hamidabadi
1
, Rafieh Alizadeh
2
, Maryam Nazm Bojnordi
1
and
Ali Niapour
3
1
Mazandaran University of Medical Sciences, Iran
2
Iran University of Medical Sciences, Iran
3
Ardabil University of Medical Sciences, Iran
P
ossible production of dopaminergic neurons fromhuman umbilical cord-derivedmesenchymal stem cells (HUC-
MSCs) is a big promise for neural tissue engineering and clinical treatment of neurodegenerative diseases, such
as Parkinson’s disease. The main goal of the current study was to determine the differentiation potential of HUC-
MSCs toward dopaminergic neuron-like cells. HUC-MSCs were isolated and cultured on Matrigel and induced
with a cocktail of dopaminergic neuron differentiation factors. The capacity of HUC-MSCs for differentiation into
dopaminergic neuron-like cells was assessed by real-time PCR, immunocytochemistry and high-performance
liquid chromatography (HPLC) and compared to the cells differentiated in cell culture plate. The differentiation
assessment at the level of mRNA and protein illustrated that Matrigel significantly increased the markers related
to dopaminergic neurons compared to the culture plate. Taken together, the results suggest that HUC-MSCs can
successfully differentiate into dopaminergic neuron-like cells on Matrigel and may have a promising potential for
treatment of dopaminergic neuron-related diseases.
Biography
Hatef Ghasemi Hamidabadi has completed her Graduation at Tehran University of Medical Sciences in 2011. He is a Member of German Neurosciences and
Immunogenetic Research Center. He has published more than 25 articles in the field of stem cells.
Hatef Ghasemi Hamidabadi et al., J Alzheimers Dis Parkinsonism 2019, Volume 09