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Volume 09
Journal of Alzheimers Disease & Parkinsonism
ISSN: 2161-0460
Epilepsy 2019
Parkinsons Congress 2019
August 29-31, 2019
JOINT EVENT
conferenceseries
.com
August 29-31, 2019 Vienna, Austria
&
5
th
International Conference on
Epilepsy & Treatment
5
th
World Congress on
Parkinsons & Huntington Disease
Therapeutic use of the curcumin and its derivative, (tetrahydrocurcumin) on MPTP induced parkinson’s
disease in male mouse
Muthukumara Sabesan
Annamalai University, India
C
urcumin (diferuloylmethane), a polyphenol extracted from the plant Curcuma longa, is widely used in food both
in India and China. It is also used in therapeutic uses. In this study we used curcumin and its derivative first time
to treat neurodegenerative disease, Parkinson’s disease (PD). In this disease the increased reactive oxygen species
(ROS) accumulation and oxidative damage of lipids, nucleic acids and proteins occur. Therapeutic use of curcumin
for this neurodegenerative disease appears multifactorial which regulates the enzymes, cytokines, monoamine
oxidase-B inhibition, and transcription factors. We investigated free radicals, enzymatic and non-enzymatic
antioxidants in on methyl 4-phenyl 1, 2, 3 4 tetra hydro pyridine (MPTP). In this model depletion of dopamine (DA)
and DOPAC (3, 4 dihydroxy phenyl acetic acid) occurs with increased activity of monoamine oxidase (MOA-B). We
used HPLC with electrochemical detection to measure DA and DOPAC respectively while MAO-B was assayed by
spectroflourimetry using the conversion of fluorogenic substrate, kyuramine. Systemic administration of curcumin
(80 mg/kg i.p) and tetrahydro curcumin (60mg/kg ip) significantly reversed the MPTP induced depletion of DA
and DOPAC. The MOA-B activity was also significantly inhibited by these compounds. The results showed that
curcumin and its derivative reversed the MPTP induced depletion of DA and DOPAC which may in part be due to
inhibition of MAO-B activity. This result also supported by free radical estimation, antioxidant assay and electron
microscopical observations. In conclusion both curcumin and its metabolite exert neuroprotection against MPTP
induced neurotoxicity.
Biography
M Sabesan has completed his PhD from Bharathidasan University and Postdoctoral studies from Freiburg University, Germany. He worked as Professor and
Head with Dean, Faculty of Science, Annamalai University, India. He worked on PD and published 20 papers in these neurodegenerative diseases in the high
impact factor journals. He guided seven students for PhD in the same field. He has completed four major research projects in this subject.
Muthukumara Sabesan, J Alzheimers Dis Parkinsonism 2019, Volume 09