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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
J Biotechnol Biomater 2017, 7:1(Suppl)
Quantitative RP-UPLC analysis of quercetin in three Grewia tenax phenotypes
Hussien M Daffalla1, S G Musharraf 2, M Iqbal Choudhary2, Mutasim M Khalfala3 and Hiba A Ali1
1National Centre for Research, Sudan
2University of Karachi, Pakistan
3Umm AL-Qura University, Saudi Arabia
Grewia tenax (Forssk.) Fiori. (Malvaceae) is commonly found in Africa, Asia and Australia. It has been used traditionally to
treat various diseases. The extracts from various plants, which are expected to be safe, exhibited various biological effects, e.g.,
anti-oxidant, antibacterial, hepatoprotective, anti-inflammatory, anti-emetic, anti-malarial, analgesic, and anti-pyretic activities.
Such effects might be attributed to the flavonoidal content of the species, e.g., quercetin. A total of 25 accessions of G. tenax were
selected for this study from trees grown within the same geographical area. Seven morphological traits were measured for each
accession. Three phenotypes were identified according to their distinct variations in leaf and stem morphology. Air dried leaves and
stem were extracted separately using 80% methanol. The methanolic extracts were fractionated sequentially using petroleum ether,
dichloromethane and ethyl acetate. Phytochemical analysis was carried out to detect variations in quercetin content in leaves and
stems within the phenotypes. A reversed-phase ultra-performance liquid chromatography, using an ultraviolet diode array detector
(RP-UPLC-UV/DAD) assay was standardized for quercetin detection and quantification in the ethyl acetate fractions. The results
showed variation in quercetin contents between different phenotypes, and between leaves and stem. The highest quercetin content
(14.09 mg/L) was present in stem of G. tenax phenotype SUST1. These results reinforce the strong phenotypic effect on the secondary
chemical profile. The variability in quercetin content in G. tenax might be related to genotypic or parent of origin effects. The clear
morphological characters variation measured in studied plants provided a good indicator to distinguish between them in quercetin
contents.