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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
J Biotechnol Biomater 2017, 7:1(Suppl)
Plant tissue culture applied biotechnology provides safe, effective and sustainable active ingredients for
skin care applications
Fabio Apone1, 2
1Vitalab srl, Italy
2Arterra Bioscience srl, Italy
The use of plants as a source of active ingredients for health care was a common practice in all the passed civilizations, and still
plant derived products have been extensively used for many types of therapeutic applications. It has been evaluated that in the
last 20 years around 40% of the newly developed compounds or extracts for human health care have been natural products or derived
from products of plant origin. Skin care research is constantly looking for new plant ingredients, which can be guaranteed for quality
and safety to final consumers, as these need to be utilized in unregulated quantities. Unfortunately, several plant derived products can
be used limitedly because it contains potential allergenic compounds, and may be subjected to environmental contaminations, such
as pollution, pesticides or agrochemical residues. To bypass these limitations, plant tissue culture techniques can provide alternative
solutions for producing valuable metabolites with skin care applications. These systems allow to cultivate plant cells or tissues in sterile
conditions, totally independent from geographical and climatic factors, and with no risk of biological nor chemical contamination.
Starting from different plant species and adopting new biotechnological approaches, we developed different types of tissue cultures,
including cell, hairy root and somatic embryo suspension cultures, and used them as sources of active ingredients for skin care
applications. The obtained ingredients, tested on skin cell cultures in vitro and on human volunteers in vivo, showed a wide range of
cosmetic and dermatological activities, ranging from UV protection, anti-inflammation, hydration to anti-ageing.
Isolation and characterization of a new Bacillus thuringiensis strain with a promising toxicity against
lepidopteran pests
Hanen Boukedi, Slim Tounsi and Lobna Abdelkefi-Mesrati
Centre of Biotechnology of Sfax, Tunisia
Bacillus thuringiensis is an aerobic, spore-forming, gram-positive soil bacterium. This microorganism produces at the sporulation
stage of its growth an intracellular crystal composed of one or more δ-endotoxins. BLB459 is a new B. thuringiensis isolated in the
laboratory of biopesticides from a Tunisian soil sample. In the present study, we focused on the screening of B. thuringiensis collection
of 200 strains isolated from different variety of Tunisian soils and the characterization, using RFLP and molecular hybridization, of B.
thuringiensis strain BLB459 having a distinctive plasmid profile. SmaI-PFGE typing confirmed the uniqueness of the DNA pattern of
this strain, compared with BUPM95 and HD1 reference strains. PCR and sequencing assays revealed that BLB459 harbored three cry
genes (cry30, cry40 and cry54) corresponding to the obtained molecular sizes in the protein pattern. Interestingly, PCR-RFLP assay
demonstrated the originality of the BLB459 cry30-type gene compared to the other published cry30 genes. Insecticidal bioassays
showed that BLB459 spore-crystal suspension was highly toxic to lepidopteran comparing with that of the commercial strain HD1
used as reference. Important histopathological effects of δ-endotoxins on the tested larvae midgut were detected, traduced by the
vacuolization of the apical cells, the damage of microvilli, and the disruption of epithelial cells. Such results indicated the interest of
the new selected B. thuringiensis strain BLB459 and their cry toxins in the biological control of different lepidopteran insects such as
lepidopteran and the possibility of its use for the formulation of new bioinsecticides.