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conferenceseries
.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Birhanu M Kinfu et al., J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
Cyanide degrading alkaliphilic bacteria from Rift Valley soda lakes and their potential for bioremediation
Birhanu M Kinfu
1
and
Amare Gessesse
2, 3
1University of Hamburg, Germany
2Addis Ababa University, Ethiopia
3Nelson Mandela Metrpolitan University, South Africa
C
yanide is a key component in mining and other applications which release up to giant lakes of toxic waste deadly to the
environment. Treatment of cyanide waste is usually done by expensive chemical methods which only converts it to less toxic
products. Bioremediation, on the other hand, is environment-friendly and more efficient alternative. The aim of this study was to
explore selected alkaline soda lakes for cyanide degrading bacteria suited for high pH bioremediation conditions. Using medium
provided with cyanide as a sole nitrogen source and pH of 10.2, 72 isolates able to degrade and utilize cyanide were found. After
preliminary screening of the isolates based on morphology, ARDRA of PCR amplified 16S ribosomal genes following restriction
digest using AluI, HaeIII and RsaI revealed the presence of nine polymorphic groups. 16S rDNA amplicon sequence analysis further
details seven distinct bacterial species from which three belonged to genus
Bacillus
, three to genus
Halomonas
, and another was more
close to an uncultured bacterium clone. Based on phylogenetic analysis, the later was more related to and clustered with
Halomonas
sub-lineages. Isolates CNA12, CNC1 and CNS10, isolated from Lakes Abijata, Chitu and Shala respectively utilized up to 99.33% of
200 mg/l cyanide and tolerated up to concentration of 600 mg/l cyanide in batch mode. While acetate was supplied as a source of
carbon for isolation, cheap molasses interestingly enhanced bacterial growth and subsequent biodegradation of cyanide. In general,
this study shows the presence of diverse cyanide utilizing alkaliphilic bacteria in the Rift Valley Soda Lakes with practical potential
for bioremediation of cyanide waste.
Biography
Birhanu M Kinfu has obtained his MSc in Biotechnology from Addis Ababa University and worked as university Lecturer for 2 years. After winning the prestigious
DAAD (German Academic Research Service) Research Grant Award under its ‘Young Academics And Scientists’ program, he is currently pursuing his Ph.D in
Germany at the University of Hamburg, Microbiology and Biotechnology Department. He has published 3 papers in reputed journals.
birhanu.kinfu@uni-hamburg.de