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Volume 7, Issue 2 (Suppl)

J Ecosyst Ecography, an open access journal

ISSN:2157-7625

September 18-20, 2017

September 18-20, 2017 Toronto, Canada

Joint Conference

International Conference on

Environmental Microbiology and Microbial Ecology

Ecology and Ecosystems

J Ecosyst Ecography 2017, 7:2 (Suppl)

DOI: 10.4172/2157-7625-C1-030

Anticandidal, antibacterial, cytotoxic and antioxidant activities of

Calendula arvensis

flowers

Abdulmalik Abudunia

Mohammed V University, Morocco

alendula arvensis

(CA) is one of the important plants used in traditional medicine in Morocco, due to its interesting chemical

composition. The present study aimed to determine the anticandidal, antioxidant and antibacterial activities, and the effects of

extracts of CA flowers on the growth of myeloid cancer cells. Also, to characterize the chemical composition of the plant. Flowers

of CA were collected based on ethnopharmacological information from the villages around the region Rabat-Khemisset, Moroccco.

The hexane and methanol extracts were obtained by soxhlet extraction, while aqueous extracts were obtained by maceration in cold

water. CA extracts were assessed for antioxidant activity using four different methods (DPPH, FRAP, TEAC and b-carotene bleaching

test). Furthermore, the phenolic and flavonoid contents were measured; also the antimicrobial activity has been evaluated by the well

diffusion method using several bacterial and fungal strains. Finally, extracts cytotoxicity was assessed using MTT test. Phytochemical

quantification of the methanolic and aqueous extracts revealed that they were rich with flavonoid and phenolic content and were

found to possess considerable antioxidant activities. MIC values of methanolic extracts were 12.5-25 mg/mL. While MIC values of

hexanolic extracts were between 6.25-12.5 mg/mL and were bacteriostatic for all bacteria while methanolic and aqueous extracts

were bactericidal. In addition, the extracts exhibited no activity on

Candida

species except the methanolic extract, which showed

antifungal activity on

Candida tropicalis

1 and

Candida famata

1. The methanolic and aqueous extracts also exhibited anti-myeloid

cancer activity (IC50 of 31 mg/mL). In our study, we conclude that the methanolic and aqueous extracts were a promising source of

antioxidant, antimicrobial and cytotoxic agents.