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.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Ouafae Pakhrou et al., J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
Population structure and genetic diversity of an endangered and endemic Moroccan tree (
Argania spinosa
L. Skeels) based on AFLP and IRAPmarkers
Ouafae Pakhrou
1
, Leila Medraoui
1
, Chaimaa Yatrib
1
, Mohammed Alami
1
, Abdelkarim Filali-maltouf
1
, Bouchra Belkadi
1
, Fouad Manda
2
, Abdelhamid El
mousadik
2
, Saad Ibn Souda-kouraichi
3
, Cherkaoui El modafar
4
and
Abderrahim Ferradous
5
1
Mohammed V University, Morocco
2
Zohr University, Morocco
3
LBM, Morocco
4
Cadi Ayyad University, Morocco
5
Forestry Research Center, Morocco
A
rgania spinosa
L., the only representative of the monotypic genus
Argania
(Sapotaceae) is a plant endemic to Morocco with
great ecological and economical values. The oil extracted from the Argan fruit possesses innumerable nutritional and cosmetic
properties. However, the area and the density of the argan forest are in continuous deterioration. In the present study, two molecular
marker techniques AFLP and IRAP were used for genetic evaluation of 130 individuals collected from 13 population locations in
south-west Morocco. A total of 477 polymorphic bands were amplified by 4 primer AFLP combinations specific to regular genome,
and 154 polymorphic bands were observed using 4 IRAP combination primers. Average polymorphic information content (PIC),
resolving power (RP) and marker index (MI) were 0.24, 12.7 and 9.40, respectively for IRAP markers, while AFLP markers showed
high resolving power (35.81) and Marker index (25.61), also the polymorphic information content was 0.21. The two-marker
techniques target different regions of the genome, which justifies the need to combine two markers in obtaining reliable estimates of
genetic diversity. Cluster analysis of the combined data revealed that 130 individuals could be divided into two sub-populations based
on STRUCTURE, UPGMA (Unweighted pair group method with arithmetic mean) clustering, and PCoA (principal coordinate
analyses). The Jaccard's genetic similarity ranged from 0.17 to 0.60 and the genetic differentiation among populations was low
(AMOVA = 19%, Gst = 0.21), indicating the possibility of gene flow between the studied populations (Nm= 1,809). These results have
an important implication for Argan tree germplasm conservation and management programs.
Biography
Ouafae Pakhrou got her Master’s degree in crop production and during her end-of-course internship she worked on the molecular characterization of cork oak
(Quercus suber L.)
using molecular markers ISSR and SSR. recently, she obtained her Doctorat in Molecular biology and plant biotechnology and worked on the
characterization of genetic diversity of an endemic tree to Morocco "Argan tree". This study aims to identify promising trees that can be used in the constitution
of a "core collection" and the establishment of conservation and management programs. This work is carried out in the Laboratory of Microbiology and Molecular
Biology at the Faculty of Sciences of Rabat University Mohamed V and financially supported by Hassan II Academy of Science and Technology (Morocco)
o.pakhrou@gmail.com