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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Chlorpyrifos-induced alterations in cell surface topography, total protein content and optimization of
key growth and degradation regulators of
Bacillus spp.
Shweta N, Jadhav SK
and
Keshavkant S
Pt. Ravishankar Shukla University, India
I
ntensive andmodern agriculture is extensively and purely based on the usage of pesticides for enhancing the crop productivity.
In India, due to warm and humid climatic condition in most of the parts the agricultural productivity is under the major
threat of variety of insects and pests. Hence, the highest consumption of pesticides is in agricultural sector. Organophosphates
are one of the widely and globally used pesticides due to its easy availability and low economic price. Continuous and repeated
use of organophosphates has become a major threat to soil environment due to its low water solubility, longer persistence and
high soil adsorption coefficient. The present study emphasized on isolation and molecular authentication of organophosphate
tolerant bacterial strains frompaddy growing field using stimulated natural environment procedure. Molecular characterization
identified the bacterial strains to be the member of
Bacillus
and
Streptomyces
genera. Scanning electron microscopy of one of
the best tolerant bacteria revealed alterations in their cell surface as well as in their length and width. Total protein content
was determined in the control sample and organophosphate (Chlorpyrifos) treated bacterial cells and accumulated data
indicated a significant impact of applied concentrations of Chlorpyrifos on content of total protein predicting the expression
of Chlorpyrifos responsive enzymes. Moreover, optimization of key growth regulators for the bacterial cell revealed that the
0.25% of Fructose and Yeast Extract was the optimum carbon and nitrogen source as well as 27.5
o
C and 8.0 were the best
temperature and pH. Even, induction of Chlorpyrifos degrading enzymes was highest at pH 8.0 and temperature 30
o
C. Thus
overall study suggested that the bacterium would be efficient and can be studied further in detail to be used for bioremediation
of Chlorpyrifos contaminated sites.
Biography
Shweta Nistala recently defended for Ph.D degree from School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, India. She is working as an
environmental microbiologist and has expertise regarding isolation of the microbes using the modern cultivation approaches. She has been testing the microbes for
biodegradation purpose of pesticides for achieving sustainability and also optimizing the growth and degradation conditions for the bacteria. She has been working
in this particular area since three years. Achievements of isolating positive and responsive pollutant (especially pesticides) degrading bacteria have been made. In
relation to this, she has published one paper and others are in communication.
shwetanistala@gmail.comShweta N et al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-038