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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Assessment of top soils formcpa utilizing bacteria isolated fromoil palmplantation and open dumpsite
Bello Osagie IO, Ekhaise FO
and
Obayagbona ON
University of Benin, Nigeria
Statement of the Problem:
MCPA (4-Chloro-2-Methylphenoxyacetic acid), one of the organochlorine herbicides is widely
used in agriculture for weed control in crops and pastures. Though it has contributed immensely in boosting agricultural
produce, its accumulation in the soil may pose potential environmental and health hazards. This work attempts to evaluate
the biodegradative potentials of indigenous MCPA utilizing bacteria in top soils of oil palm plantation and open dumpsite as a
natural way to control toxic accumulation of MCPA.
Methodology and Theoretical Orientation:
Standard bacteriological methods were utilized in the isolation of heterotrophic
bacterial flora, physico-chemical procedures were used for the evaluation of soil profile while molecular techniques targeting
the 16S rRNA of isolates were utilized in characterization of MCPA utilizing bacteria.
Findings:
The mean total bacterial counts from the sampled sites were not significantly different (P>0.05). The growth profile
of the axenic and the mixed bacterial cultures were evaluated using parameters such as pH, optical density, chloride release
and bacterial count for 15 days. Of the Six (6) bacterial species characterized as
Bacillus atrophaeus, B.pumilis, B.licheniformis,
B.subtilis,Achromobacter xylosoxidans
and
Pseudomonasmendocina,
only three (3) species
(Bacillus licheniformis,Achromobacter
xylosoxidans
and
Pseudomonas mendocina)
exhibited biodegradative potential for MCPA in the soil samples. Physico-chemical
analysis of soil samples revealed slightly acidic (5.2-6.9) and sandy (89.0-95.0) profile, with ammonia-nitrogen and phosphate
values ranging from 6.12 mg/kg to 17.80 mg/kg and 19.09 mg/kg to 56.23 mg/kg respectively. The biodegradation of MCPA
was greatly enhanced with the use of consortium of the isolates as indicated by the comparatively higher values of mean colony
counts and chloride release recorded in flasks which contained glucose as extra energy and carbon source.
Biography
Idowu Bello-Osagie is a Lecturer in the Department of Microbiology, Faculty of Life sciences, University of Benin. Her research interest spans the Environmental
sector of Microbiology with focus on water quality, management and technology. She has worked extensively on biodegradation and enhanced remediation of
endocrine disrupting compounds (bisphenol A) as well as herbicide degrading bacteria. She has evaluated the ecological implications of anthropogenic pollutants
in soil and aquatic ecosystems as well as antibiograms and mutagenicity assessment of hospital waste water. Hundreds of students have been positively mentored
over years of serving as academic advisor and teacher. She has actively participated in various local, national and international conferences, presenting scientific
papers as necessary.
idowu.bello-osagie@uniben.eduBello Osagie IO al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-038