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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8

July 11-12, 2018 | Toronto, Canada

International Conference on

Environmental Microbiology & Microbial Ecology

International Conference on

Ecology, Ecosystems & Conservation Biology

&

Pichia

kudriavzevii

a NN/SG II potential yeast strain isolated from Gora- a rice based fermented beer

traditionally prepared by the indigenous Koloi tribes of Tripura

Nandita Nath, Sushanta Ghosh, Lovely Rahaman, Senthil Kumar Nachimuthu

and

Bipin Kumar Sharma

Tripura University, India

G

ora a rice based fermented beer traditionally prepared by the Koloi tribes of Tripura using indigenous brewing techniques.

The present study aimed to isolate and identify the indigenous yeast flora of ‘Gora’ and evaluate its fermentation ability

comparison with a industrial strain. Culture dependent approaches- phenotypic characterization were done to investigate the

responsible yeast species and identified as

Pichia

kudriavzevii

which was further confirmed by the molecular identification

using partial 18S rRNA gene sequence. The reducing and non-reducing sugar content of ‘Gora’ was 1.19 ± 0.999 and 1.04 ±

0.010 mg/ml respectively. Whereas the carbohydrate, protein and Total alcohol content of the rice beer ‘Gora’ was 0.56 ±0.101,

11.96 ± 0.001 mg/ml and 6.40 ± 0.008 % v/v. respectively. The comparative analysis of the isolate

Pichia

kudriavzevii

a NN/SG

II with the industrial strains in laboratory condition shows significant similarities in the nutritional parameters and alcohol

percentages with the industrial strain. This is the first scientific investigation of indigenous yeast strain of ‘Gora’ from this region

may provide sufficient background and potentiality for its scientific and logical appreciation and promoting the microbial

legacy of indigenous alcoholic beverage as an immaterial cultural heritage for future as well as small scale commercialization

for rural livelihood.

Biography

Nandita Nath is a Research Scholar at the Tripura University, India. She has extended his valuable service for many years and has been a recipient of many award

and grants. Her international experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research

interests reflect in his wide range of publications in various national and international journals.

naina5ieee@gmail.com

Nandita Nath et al., J Ecosys Ecograph 2018, Volume 8

DOI: 10.4172/2157-7625-C3-038