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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Pichia
kudriavzevii
a NN/SG II potential yeast strain isolated from Gora- a rice based fermented beer
traditionally prepared by the indigenous Koloi tribes of Tripura
Nandita Nath, Sushanta Ghosh, Lovely Rahaman, Senthil Kumar Nachimuthu
and
Bipin Kumar Sharma
Tripura University, India
G
ora a rice based fermented beer traditionally prepared by the Koloi tribes of Tripura using indigenous brewing techniques.
The present study aimed to isolate and identify the indigenous yeast flora of ‘Gora’ and evaluate its fermentation ability
comparison with a industrial strain. Culture dependent approaches- phenotypic characterization were done to investigate the
responsible yeast species and identified as
Pichia
kudriavzevii
which was further confirmed by the molecular identification
using partial 18S rRNA gene sequence. The reducing and non-reducing sugar content of ‘Gora’ was 1.19 ± 0.999 and 1.04 ±
0.010 mg/ml respectively. Whereas the carbohydrate, protein and Total alcohol content of the rice beer ‘Gora’ was 0.56 ±0.101,
11.96 ± 0.001 mg/ml and 6.40 ± 0.008 % v/v. respectively. The comparative analysis of the isolate
Pichia
kudriavzevii
a NN/SG
II with the industrial strains in laboratory condition shows significant similarities in the nutritional parameters and alcohol
percentages with the industrial strain. This is the first scientific investigation of indigenous yeast strain of ‘Gora’ from this region
may provide sufficient background and potentiality for its scientific and logical appreciation and promoting the microbial
legacy of indigenous alcoholic beverage as an immaterial cultural heritage for future as well as small scale commercialization
for rural livelihood.
Biography
Nandita Nath is a Research Scholar at the Tripura University, India. She has extended his valuable service for many years and has been a recipient of many award
and grants. Her international experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research
interests reflect in his wide range of publications in various national and international journals.
naina5ieee@gmail.comNandita Nath et al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-038