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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume 8
July 11-12, 2018 | Toronto, Canada
International Conference on
Environmental Microbiology & Microbial Ecology
International Conference on
Ecology, Ecosystems & Conservation Biology
&
Antiproliferative anthracycline pink red-like pigments produced by new bacterial soil strains identified
as
Streptomyces coelicoflavus
and bioactivity of other compounds
M. Menggad
1
, A Mouslim, H Ayoubi, N Habti, S Menggad
2
, S Moujabbir
and
E. Affar
1
University Hassan II Casablanca, Morocco
2
University of Montreal, Canada
A
mong 29 soil isolated actinomycetes, five new strains MFB11, MFB20, MFB21, MFB23 andMFB24 showed an intracellular
hydrophobic pink red-like pigment production. These pigments present similar physio-chemical characteristics with
anthracycline antibiotics of prodigiosin family. Crud extract and prepared fractions were tested by MTT on mice cancer cell
line as well on human cancer cell line. The results indicated an important antiproliferative effect of the different strain pigments
on the two organism cell types. Human cells were more sensitive to the pigments and presented different antiproliferative effect
profiles. FACs analysis of this antiproliferative effect on cancer human cells line showed a cell cycle phase arrests at G1 and S.
Nevertheless, negative antibacterial assay, Thin-layer chromatography (TLC) and interaction with organic solvents analysis of
these pigments revealed their difference from known anthracycline antibiotics. Morphological, biochemical and gene coding
16S RNA sequence analysis allowed identification of the producer strains as
Streptomyces coelicoflavus
; known to produce
important aminoglycoside antibiotics and other bioactive compounds but not anthracycline red-like pigments. Otherwise, two
other strains produced water soluble Gram positive antibiotics and chloroform soluble bioactive compounds with strong and
dramatic apoptotic antiproliferative activity as indicated by MTT and their cell cycle phase arrests at G0/G1 and G2.
Biography
Mohammed Menggad is a Professor in Hassan-II University of Casablanca, Morocco. He has completed Graduate diploma from Mohammed-V University, Rabat,
Morocco. Postgraduate diploma and PhD from Paris XI University, France. He has experiences at Max-Planck-Institut fur Zellbiologie, Rosenh of Ladenburg,
Germany and at Queen's University, Department of Biomedical and Molecular Sciences, Kingston, Canada.
mengm106@yahoo.frM. Menggad et al., J Ecosys Ecograph 2018, Volume 8
DOI: 10.4172/2157-7625-C3-038