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conferenceseries.com Joint Conference
July 17-18, 2017 Chicago, USA
International Conference on
DIAMOND AND CARBON MATERIALS & GRAPHENE AND SEMICONDUCTORS
Volume 6, Issue 6 (Suppl) J Material Sci Eng, an open access journal
ISSN: 2169-0022
Diamond and Carbon 2017 & Graphene 2017
July 17-18, 2017
Mass production of functionalized graphene quantum dots and their application in cancer
diagnosis and therapy
Yanli Wang, Chenjie Yao, Lin Ding, Chenchen Li, Yanan Huang and Minghong Wu
Shanghai University, China
Graphene quantum dots (GQDs) have garnered increasing attention because of their various alluring physicochemical
properties and a wide range of potential application. However, their large-scale applications are limited by current synthetic
methods that commonly produce GQDs in small amounts. Moreover, GQDs usually exhibit polycrystalline or highly defective
structures and thus poor optical properties. We established a new mass-productive synthesis method of single-crystalline
GQDs via a green and low-cost alkali-mediated hydrothermal molecular fusion using an active PAH molecule as a precursor.
Functionalized GQDs featured excellent optical properties, strong excitonic absorption bands extending to the visible region,
large molar extinction coefficients, and long-term photo-stability. Positively charged amino-GQDs could easily pass through
cell membranes and localize in cell cytoplasm but show acute toxicity in vivo causing death of mice even after tail vein injection
at low concentrations. Negatively charged sulfonic-GQDs could not pass through the cell membrane even after co-incubation
for 48 hours under normal culture conditions. However, specific targeting of cell nuclei was found when cells were cultured in
an ultrathin film with the sulfonic-GQDs. Both subcutaneous and orthotopic mice tumor models revealed that the sulfonic-
GQDs showed specific targeting performance for the tumor tissues in vivo. Confocal images of frozen tumor and normal tissue
sections further demonstrated the exceptional in vivo cancer-cell-nuclear-targeting capability of the sulfonic-GQDs, which
showed no cancer type specificity. Both materials showed good performance for both in vitro and in vivo imaging, however, the
toxicity issues of the amino-GQDs may limit their in vivo applications. Negatively charged sulfonic-GQDs showed low toxicity
both in vitro and in vivo, which indicates their good potential for clinical applications.
Biography
Yanli Wang is currently working at Institute of Nanochemistry and Nanobiology of Shanghai University as Associate Professor since 2012. She obtained her PhD
degree in Environmental Engineering from Shanghai University in 2010. Her research interests mainly focus on bio-effects and safety evaluation of nanomaterials
and their application in bio-imaging and cancer therapy..
Yanli Wang et al., J Material Sci Eng 2017, 6:6(Suppl)
DOI: 10.4172/2169-0022-C1-076