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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
A multiparametric analysis of the synergistic impact of anti-Parkinson's drugs on the fibrillation of
human serum albumin
Tajalli Ilm Chandel
1
, Nida Zaidi
1
, Masihuz Zaman
1
, Ishrat Jahan
3
, Aiman Masroor
1
, Ibrar Ahmad Siddique
2
, Shahid M Nayeem
3
, Maroof Ali
4
, Vladimir
N Uversky
5, 6, 7
, Rizwan Hasan Khan
1
1
Interdisciplinary Biotechnology Unit-Aligarh Muslim University, India
2
National Institute of Immunology, India
3
Aligarh Muslim University, India
4
Moradabad Institutes of Technology, India
5
Institute for Biological Instrumentation-RAS, Russia
6
King Abdulaziz University, Saudi Arabia
7
USF Health Byrd Alzheimer's Research Institute-University of South Florida, USA
P
rotein aggregation has been associated with several human neurodegenerative diseases, such as Parkinson's and
Alzheimer's diseases. There are several small molecules that can reduce aggregation of proteins. The present study aimed to
test the hypothesis that the application of more than one inhibitor either simultaneously or consecutively may result in more
efficient inhibition of protein aggregation. To this end, the anti-amyloidogenic behaviour of benserazide hydrochloride (BH)
and levodopa (LD) individually and in combination (BH + LD) was investigated using various biophysical, microscopic and
computational techniques. BH, LD, and BH+LD treatments showed inhibitory effects on protein aggregation and had the ability
to minimize the amyloid-induced cytotoxicity in human neuroblastoma cell line (SH-SY5Y). The two drugs in combination
showed synergism (combination index, CI < 1) between them. These drugs also destabilized the preformed fibrils of human
serum albumin (HSA). Our studies consistently showed that the BH+LD treatment showed highest efficacy towards inhibition
and disaggregation of amyloid fibrils in comparison to treatment with BH and LD individually. Therefore, application of drugs
in combination against fibrillogenesis may represent a new route for the development of means for prevention or delaying of
the aggregation-related diseases.
tajalli_chandel@yahoo.co.inJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-116