

Page 70
conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
Epidemiology of duck as reservoir of avian influenza virus in Bangladesh
Md. Zakir Hassan
1, 2
1
National Reference Laboratory for Avian Influenza (OIE Reference Laboratory), Bangladesh
2
Livestock Research Institute, Savar, Dhaka, Bangladesh
T
his research was conducted to detect the prevalence and incidence of avian influenza virus (AIV) in duck at hoar area of
Kishorganj in Bangladesh in 2013. A total of 736 serum and 150 cloacal samples were collected from asymptomatic semi
scavenging duck above four months of age. The serum sample was tested for AIV specific antibody through cELISA and cloacal
sample was tested to detect the incidence of AIV through RT-PCR in CDIL, Dhaka. Total of 684 is positive (prevalence) (+ve)
of Avian Influenza type A antibody. The prevalence rate was 92.93%. The tested result shown that prevalence rate was at 4-6
months of age as 93.96% and 7-9 month of age as 92.92 % and in 10-12 month of age as 91.91%. A total number of 15 pooling
samples from 75 cloacal samples was conducted for detection of AIV that shed in environment. After calculating the result
through RT-PCR, it was shown that two pooling samples was positive (incidence) (13.33%) for AIV. Epidemiology of this hoar
duck can be shown that they can transmit the AIV in the surrounding poultry population and clinical outbreaks may occur. To
sum-up the result of ELISA and PCR, it was illustrated that duck act as a natural reservoir of AIV in Bangladesh.
zakir.vet@blri.gov.bd zhtitas@gmail.comJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-116