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Volume 9

Journal of Biotechnology & Biomaterials

ISSN: 2155-952X

JOINT EVENT

February 28-March 02, 2019 | Berlin, Germany

5

th

International Conference on

Enzymology and Protein Chemistry

&

22

nd

Global Congress on

Biotechnology

Biotechnology 2019

Enzymology 2019

February 28-March 02, 2019

&

Epidemiology of duck as reservoir of avian influenza virus in Bangladesh

Md. Zakir Hassan

1, 2

1

National Reference Laboratory for Avian Influenza (OIE Reference Laboratory), Bangladesh

2

Livestock Research Institute, Savar, Dhaka, Bangladesh

T

his research was conducted to detect the prevalence and incidence of avian influenza virus (AIV) in duck at hoar area of

Kishorganj in Bangladesh in 2013. A total of 736 serum and 150 cloacal samples were collected from asymptomatic semi

scavenging duck above four months of age. The serum sample was tested for AIV specific antibody through cELISA and cloacal

sample was tested to detect the incidence of AIV through RT-PCR in CDIL, Dhaka. Total of 684 is positive (prevalence) (+ve)

of Avian Influenza type A antibody. The prevalence rate was 92.93%. The tested result shown that prevalence rate was at 4-6

months of age as 93.96% and 7-9 month of age as 92.92 % and in 10-12 month of age as 91.91%. A total number of 15 pooling

samples from 75 cloacal samples was conducted for detection of AIV that shed in environment. After calculating the result

through RT-PCR, it was shown that two pooling samples was positive (incidence) (13.33%) for AIV. Epidemiology of this hoar

duck can be shown that they can transmit the AIV in the surrounding poultry population and clinical outbreaks may occur. To

sum-up the result of ELISA and PCR, it was illustrated that duck act as a natural reservoir of AIV in Bangladesh.

zakir.vet@blri.gov.bd zhtitas@gmail.com

J Biotechnol Biomater 2019, Volume 9

DOI: 10.4172/2155-952X-C2-116