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.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
Genetic diversity analysis of lentil (Lens culinaris Medikus culinaris) accessions having high
concentration of iron (Fe) and zinc (Zn) using by simple sequence repeat (SSR) markers
Rajendra Darai
1
, Ashutosh Sarker
2
, Krishna Hari Dhakal
3
, Madhav Prasad Pandey
3
, Surya Kant Ghimire
3
, Shiv Kumar
4
, Tara B Ghimire
5
, Jitendra
Maharjan
5
and
Laxman Aryal
1
1
Nepal Agricultural Research Council, Nepal
2
ICARDA, India
3
Agriculture and Forestry University, Nepal
4
International Center for Agricultural Research in the Dry Areas, Morocco
5
Seed Science Research and Technology Division- NARI, Nepal
N
epalese lentils are comparatively rich in iron (Fe) and zinc (Zn) in South Asian countries, making lentil a potential crop of
whole food solution to aid in the global battle against the micronutrient malnutrition. Knowledge of genetics underlying
the uptake of grain Fe and Zn from soils is required to increase their stable concentrations in lentils. Therefore, in present
study, 25 accessions of lentil were characterized using 40 simple sequence repeat (SSR) markers in order to characterize genetic
variation available among genotypes having high Fe and Zn concentrations. Out of the 40 SSR markers, 23 markers were found
polymorphic while 12 markers were monomorphic and 5 markers were null. These 23 polymorphic markers produced a total
of 584 alleles, of which total number of polymorphic alleles were 52 and average alleles per locus was 11.49. The allele number
for each SSR locus varied between two to four with an average of 2.97 alleles per marker. Markers PLC 16, SSR 124, SSR 156,
SSR 113, SSR 28 and SSR 107 showed higher level of polymorphism indicating the power and higher resolution of those marker
systems in detecting molecular diversity. The polymorphic information content (PIC) values for the SSRs loci ranged from 0.14
to 0.57. The pair wise genetic similarity among 25 lentil accessions varied from 0.16 to 0.83. The dendrogram constructed based
on genetic similarities among 25 lentil accessions identified five major clusters. Maximum seven accessions were grouped in
cluster II followed by six in cluster III while cluster IV contained lowest number of accessions i.e. three accessions indicating
their higher genetic similarity. Our result showed that significant genetic variability at molecular level on the basis of SSR
markers that can be used towards the development of lentil cultivars having high concentration of Fe and Zn.
rajendra5042@yahoo.co.ukJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-116