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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
Genetic and transcriptomic analyses of disease response to Botrytis cinerea in Arabidopsis thaliana
Synan AbuQamar
United Arab Emirates University, UAE
T
ranscriptional reprogramming forms a major part of a plant’s response to environmental stress. We investigated the
effects of combinations of biotic and abiotic stresses on the transcriptome level of Arabidopsis genome using comparative
microarrays. We showed a unique program of gene expression was activated in response to each biotic and abiotic stress. In
addition, abiotic stress-induced genes were commonly regulated with Botrytis cinerea infection. The Arabidopsis cell wall
expansin-like A2 (EXLA2) gene was identified based on its down-regulation in response to infection by the necrotrophic
pathogen B. cinerea, and on the reduced susceptibility of its mutants to the same pathogen. The exla2 mutants also enhanced
tolerance to the phytoprostane-A1 (PPA1). Our results suggest that the absence or down-regulation of EXLA2 leads to
increased resistance to B. cinerea in a COI1-dependent manner, and this down-regulation can be achieved by PPA1 treatment.
The EXLA2 is significantly induced by salinity and cold, and exogenous application of abscisic acid (ABA). The exla2 mutant
also showed hypersensitivity towards increased salt and cold, and this hypersensitivity required a functional ABA pathway.
Overall, EXLA2 appears to be important in response to environmental stress, particularly in the pathogenesis of necrotrophic
pathogens and tolerance to abiotic stress. Future directions to further analyze the functions of commonly expressed genes in
response to environmental stress will increase our understanding of the plant stress response.
sabuqamar@uaeu.ac.aeJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-116