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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20
th
Global Congress on
Biotechnology
3
rd
International Conference on
Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Solid-phase extraction of protein by using magnetic chitosan and graphene oxide-functional
guanidinium ionic liquid composite
Xueqin Ding
Hunan Normal University, China
A
series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium
ionic liquids have been synthesized. Magnetic chitosan-graphene oxide (MCGO) composite has been prepared and
coated with the functional guanidinium ionic liquids for protein extraction. Vibrating sample magnetometer, x-ray diffraction
spectrometer, field emission scanning electron microscopy and Fourier transform infrared spectrometer were used to
characterize the MCGO-functional guanidinium ionic liquid composite. After extraction, concentrations of protein were
determined by an ultra violet visible spectrophotometer. Lysozyme, trypsin, BSA and ovalbumin could be well extracted by
the proposed MCGO-functional guanidinium ionic liquid magnetic solid-phase extraction system. Compared to magnetic
chitosan, graphene oxide, MCGO and MCGO-ordinary imidazolium ionic liquid, the MCGO-functional guanidinium ionic
liquid has higher extraction capacity. Single factor experiments proved that the extraction efficiency of protein was affected by
temperature, pH value, extraction time, amount of MCGO-functional guanidinium ionic liquid composite and concentration
of protein. Moreover, the MCGO-functional guanidinium ionic liquid can be easily regenerated; the extraction capacity after
being used three times was 94% of the first time.
xueqinding@hunnu.edu.cnJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-092