biotech-congress-enzymology-2018-posters-abstracts

19 / 22

Page 89
conferenceseries.com
Volume 8 Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20th Global Congress on Biotechnology
3rd International Conference on Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Studies on optimization of improved amylases developed by protoplast fusion of Aspergillus species
Tolulope Modupe Adeleye1 , Sarafadeen Olateju Kareem1, Bankole Mobolaji Olufunmilayo1, Olusegun Atanda2, Michael Bamitale Osho2 and Olawale Dairo1
1Federal University of Agriculture Abeokuta, Nigeria
2McPherson University, Nigeria
Improved amylase producers were developed by the fusion of protoplasts from two indigenous amylase-producing Apergillus
species. Twenty randomly selected, regenerated fusants were screened for improved amylase activities. Selected fusnats were
used for further comparative studies with the parent isolates. Effect of incubation period on amylase activities of parent and
fusant isolates were studied by assay of crude enzymes produced by solid state fermentation on rice bran and used for cassava
starch hydrolysis. The interaction between temperature, pH and enzyme types (parental and selected fusants) was studied and
optimized using the central composite design (CCD) of the response surface methodology (RSM). Fusant designates, T5 T13
and T14 were selected for improved activities over parents and were used in further comparative studies. Assay of amylase
activities of randomly selected regenerated fusants at room temperature and at 80ºC showed designates, T5 (920.21 U/ml,
966.67 U/ml), T13 (430 U/ml, 1011.11 U/ml) and T14 (500.63 U/ml, 1012.00 U/ml) as preferred fusants. Amylases produced
by the fusants were observed to be active over the range of pH studied. Analysis of the sequences generated suggests the
occurrence of genetic recombination in selected fusants, fusants T13 and T14 had optimum amylase production at 72 hours,
while parents TA, TC and fusant T5 produced amylase maximally after 96 hours of incubation. Optimum amylase activity was
observed from amylase from fusant T5 at pH 4 and temperature of 40ºC. These results show the efficiency of protoplast fusion
technique in strain development for improved amylase activity.
J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-092