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Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
Hyaluronanmicroenvironment enhances chondrogeneic but prevents osteogenic effects of simvastatin
in adipose derived stem cells
Je-Ken Chang, Shun-Cheng Wu, Chung-Hwan Chen, Jhen-Wei Chen, Che-Wei Wu, Chien-Hsueh Chen
and
Mei-Ling Ho
Kaohsiung Medical University, Taiwan
S
imvastatin (Sim) is a clinically used lipid lowing agent, which has been indicated to increase bone morphogenetic protein-2
(
BMP-2
) expression.
BMP-2
has been demonstrated to play a critical role in cartilage development, and is applied to promote
chondrogenic differentiation of mesenchymal stem cells (MSCs). However,
BMP-2
was also used for osteogenic differentiation
of MSCs. Hyaluronan (HA) is one of the main extra cellular matrices during the early stage of chondrogenesis, and we
previously found that HA microenvironment initiates and promotes chondrogenesis of adipose derived stem cells (ADSCs). In
this study, we hypothesize that HA enhances the chondrogenic effect and prevents osteogenic effect of Sim on ADSCs, and this
can be applied for cartilage regeneration. The ADSCs were cultured
in vitro
in HA-coating, well treated with Sim, and the
BMP-
2
expression, chondrogenesis as well as osteogenesis of ADSCs were analyzed. The result showed that HA microenvironment
enhances Sim-induced
BMP-2
expression and chondrogenesis of ADSCs. The real-time PCR results showed that the
BMP-
2
and chondrogenic genes (
SOX-9, Collagen type II and Aggrecan
) expressions induced by Sim in ADSCs were enhanced
when cultured in HA-coated well (Figure. 1). The result from sGAG synthesis of ADSCs also shows HA microenvironment
enhances more pronounced sulfated glycosaminoglycan (sGAG) synthesis of ADSCs that is induced by Sim (Figure. 2). HA
microenvironment also reduces osteogenic genes expressions (osteocalcin and alkaline phosphatase) and calcium deposition
of ADSCs that is induced by Sim (Figure. 3). The synergic effect of HA with Sim can promote chondrogenesis and prevent
osteogenesis of ADSCs, and may be applied for articular cartilage defect regeneration. This result shows the local cue on
affecting chondrogenesis and osteogenesis of
BMP-2
in ADSCs induced by Sim.
Figure 1. The mRNA expression levels of BMP-2 and chondrogenic genes of ADSCs in the Control (Non-treated), Sim (Sim treatment), HA (HA-coated well) and HA+Sim
(HA-coated well with Sim treatment) groups on day 1, 3 and 5. Figure 2. Alcian blue staining of sGAG in 4 groups on day 7. Blue: Alcian blue staining. The sGAG synthesis
by ADSCs in 4 groups were quantified by a DMMB assay. The sGAG synthesis normalized to the total DNA in each group is expressed as sGAG/DNA. Figure 3. The mRNA
expression levels of osteogenic genes of ADSCs in 4 groups on day 1, 3 and 5. The calcium deposition by ADSCs in 4 groups on day 12. Red: Alizarin Red S staining
Je-Ken Chang et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-088