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conferenceseries
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Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
Fabrication of thermo-responsive surfaces with nanocone array
Qianqian Cui, Tianqing Liu, Kedong Song, Dan Ge, Shui Guan
Dalian University of Technology, China
Statement of the Problem:
The bacterial adhesion to the solid surfaces and subsequent formation of biofilm are greatly harmful
for the efficiency of material application and related processes. It is of important significance for protecting the environment
and life health to study the formation mechanism of biofilm and the way of preventing the formation of biofilm. At present,
we are working on fabricating the thermo-responsive material surfaces with nanocone array to regulate the bacterial adhesion
and prevent biofilm formation, i.e. Bacterial cells are penetrated and killed by nanocone structures and thermo-responsive poly
(N-isopropylacrylamide) (PNIPAAm) detaches and releases the dead cells and debrises through the change of temperature.
Method & Technology:
We have prepared hexagonally packed ordered alumina inverse taper-nanopores by using one-step hard
anodizing and etching peeling of high purity aluminum foils followed bymultistepmild anodizing and etching pore-widening.Then
adopting template-based hot embossing copies the shapes of anodic aluminum oxide template onto the polymer surfaces on which
the nononipple structures can be attained. These nononipples were transformed to nanocones by chemical dissolution. According
to the type of substrate materials, suitable methods of grafting thermo-responsive poly (N-isopropylacrylamide) (PNIPAAm) were
chosen to obtain the thermo-responsive materials with nanocone array. For example, the glass surface and polymer surface with
nanocone array were grafted with PNIPAAm by the method of ARGET-ATRP and ultraviolet irrigation, respectively.
Conclusion & Significance:
Modulating the structural parameters of the thermo-responsive nanocone surfaces will realize the
target of regulating bacterial adhesion, killing bacteria and releasing dead cells and their cell fragments. The material that is able
to regulate bacterial adhesion and prevent biofilm formation belongs to physical environmental friendly materials, which provides
new mentality for the control of bacterial adhesion and biofilm formation.
Figure 1: Schematic diagram of the dynamic evolution process of the bacteria which adhered to the temperature responsive surface with nanocone array. Green represents
PNIPAAm. 1. Bacteria attach to the temperature responsive surface with nanocone structures. 2. The cell wall of bacterium is penetrated and killed by nanocone. 3. The dead
cells break up into debris which is released from surface when changing temperature
Recent publications
1. Ivanova EP, Hasan J,WebbHKandTruongVK (2012)Natural bactericidal surfaces: mechanical rupture of Pseudomonas
aeruginosa cells by cicada wings. Small 8:2489-94.
2. Lee W, Ji R, Gosele U and Nielsch K (2006) Fast fabrication of long-range ordered porous alumina membranes by hard
anodization. Nature Materials 5:741-747.
Qianqian Cui et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-088