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Volume 8
Journal of Biotechnology and Biomaterials
ISSN: 2155-952X
Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3
rd
Annual Conference and Expo on
Biomaterials
Preparation of PVDF-g-PNIPAAm thermo-sensitive fiber membranes by electrostatic spinning and
application in cultivation and harvest of cells
Tianqing Liu, Kedong Song, Dan Ge and Shui Guan
Dalian University of Technology, China
T
rypsin digestion has long been the main way to harvest anchorage-dependent cells. But trypsin will damage the proteins of
extracellular matrix, leading to the degradation of the structure and function of cells. Cultivating cells on thermo-sensitive
material, then harvesting cells by lowering temperature, can make the extracellular matrix maintain integrity. In this study, a
series of PVDF-g-PNIPAAm thermo-sensitive fiber membranes, M21, M43, M11, and M45 were prepared by electrospinning.
Fourier transform infrared spectroscopy and NMR-H spectrum were used to characterize that the PNIPAAm was grafted
successfully. The morphology of each fiber membrane was observed by scanning electron microscope, indicating that the
grafting percentage of PNIPAAm influenced the spinnability of PVDF. Fourier transform infrared spectroscopy of PVDF and
PVDF-g-PNIPAAm fiber membranes showed that electrostatic spinning would not change the structure of thermo-sensitive
polymer. PC12 cells were seeded on the surfaces of M21, M43 andM11 for cell experiments. The cell adhesion, proliferation and
growth on different fiber membranes were examined. Then the harvested cells on different fiber membranes with temperature
reduction were compared with those harvested by trypsin digestion method. The images of cell live/dead fluorescence staining
showed that cells in different fiber membranes all had a high viability and the prepared thermo-sensitive fiber membranes had
good biocompatibility. The MTT results showed that the nanometer fiber structure was conducive to the proliferation and
growth of cells. The cells grown on the surface of M21 were the best and relatively poor on the M11, which indicated that higher
grafting ratio is not suitable for the adhesion and growth of cells. Finally the cells cultivated on the three thermo-sensitive fiber
membranes after three days were harvested with temperature reduction, showing that high grafting ratio is advantageous to the
detachment of cells. Compared with trypsin digestion method, the temperature reduction method has significant advantages.
Recent Publications
1. KatoA,KanK,AjiroH, et al. (2017)Development of a rapid
invitro
tissuedeadhesionsystemusing the thermoresponsive
sol-gel transition of hydroxybutyl chitosan. Journal of biomaterials science polymer edition 1:16.
2. Nagase K, Sakurada Y, Onizuka S, et al. (2017) Thermoresponsive polymer-modified microfibers for cell separations.
Acta biomaterialia 53:81-92.
3. Sudo Y, Sakai H, Nabae Y, et al. (2016) Role of hyperbranched polystyrene on thermo-responsive cell culture dishes
prepared by hyper branched polystyrene-g-poly (N-isopropylacrylamide). Polymer 100:77-85.
4. Mellati A, KiamahallehMV, Madani S H, et al. (2016) Poly(N-isopropylacrylamide) hydrogel/chitosan scaffold hybrid
for three-dimensional stem cell culture and cartilage tissue engineering. Journal of Biomedical Materials Research
Part A 104(11):2764-2774.
5. Sudo Y, Sakai H, Nabae Y, et al. (2015) Preparation of hyperbranched polystyrene-g-poly(N-isopropylacrylamide)
copolymers and its application to novel thermo-responsive cell culture dishes. Polymer 70:307-314.
Tianqing Liu et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-089