biochemistry-2016_posters-accepted-abstracts

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Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 45
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
Regulation of protein related antimicrobial activity by recombinant mussel adhesive protein (fp-151)
using proteome analysis
Kyung Bae Pi1, Sunhye Lee2, Sunggil Park 2, Ho-Jin Kim1, Beom-Seop Rho 1, Ki Beom Lee1, Yoonjin Lee3 and Jung-Mo Ahn1
1Incheon Business Information Technopark, Korea
2Kollodis Biosciences, Korea
3Somang Cosmetics Corporation, Korea
In this study, the antimicrobial activity of recombinant mussel adhesive protein (fp-151) was evaluated against skin flora. The skin
flora used for experiments were four Gram-positive bacteria such as Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S.
pyogenes), Staphylococcus epidermis (S. epidermis), Propionibacterium acnes (P. acnes) and one Gram-negative bacteria (E. coli). Pour
plate assay was applied for determining the antimicrobial effects of recombinant MAP (fp-151). According to the result, recombinant
MAP (fp-151) showed 90% of antimicrobial activity and was as good as or even better than penicillin for five skin flora. Also, we
aimed to identify key proteomic changes in a HaCaT cell line grown in treated with recombinant MAP (fp-151) peptide, applying
proteomic methods using nano-LC and a 4800 Plus MALDI TOF/TOF tandem mass spectrometry device. In a narrowed down
and comparative data analysis of both non-treated and treated recombinant MAP (fp-151) peptide groups, differentially expressed
proteins were identified as up or down-regulated. Bioinformatic analysis was used to reveal the biological functions and predict its
possible mechanism. These results indicate that recombinant MAP (fp-151) has a high antibacterial effect and resistance against
bacterial infection and cell damage which therefore, implies that recombinant MAP (fp-151) has its potential use in skin care products.
Biography
Kyung Bae Pi has completed his MS from Kyung Hee University. He is a Senior Researcher and Project Leader of Incheon Business Information Technopark. He has
published more than 13 papers in reputed journals and has been serving as an Editorial Board Member of repute.
Kyung Bae Pi et al., Biochem Anal Biochem 2016, 5:3(Suppl)