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Volume 5, Issue 3(Suppl)

Biochem Anal Biochem 2016

ISSN: 2161-1009, Biochem an open access journal

Page 44

Notes:

Biochemistry 2016

October 10-12, 2016

conferenceseries

.com

Biochemistry

October 10-12, 2016 Kuala Lumpur, Malaysia

International Conference on

Isolation and expression analysis of a cDNA encoding for phosphoenolpyruvate carboxylase in

thermogenic skunk cabbage,

Symplocarpus renifolius

Md Abu Sayed, Yui Umekawa

and

Kikukatsu Ito

Iwate University, Japan

S

kunk cabbage,

Symplocarpus renifolius

, is known to produce enough heat to melt the snow in early spring. We have isolated a

cDNA that encodes a phosphoenolpyruvate carboxylase, termed as

SrPEPC

from thermogenic florets of skunk cabbage.

SrPEPC

is a 110.5 kDa protein that contains conserved amino acid regions such as phosphorylation and catalytic domains. Expression of

SrPEPC mRNA was highly abundant in the petal and pistil of thermogenic florets. Interestingly, the expression was very low in non-

thermogenic tissues including spathe and leaf. Our data suggest that

SrPEPC

plays a role for tissue-specific heat production in the

skunk cabbage.

Biography

Md Abu Sayed is currently pursuing his PhD from United Graduate School of Agriculture, Iwate University, Japan. He has been serving as an Assistant Professor in the

Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh since 16 May, 2012.

u0414002@iwate-u.ac.jp

Md Abu Sayed et al., Biochem Anal Biochem 2016, 5:3(Suppl)

http://dx.doi.org/10.4172/2161-1009.S1.006