biochemistry-2016_posters-accepted-abstracts

3 / 20

Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 42
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
The role of pyruvate kinase (PK) and glucokinase (GCK) in Streptococcus mutans mixed biofilm
development
Wirginia Krzysciak, Palina Vyhouskaya, Pawel Krzysciak, Jakub Piatkowski, Anna Skalniak, Anna Jurczak, Dorota Koscielniak and Ryszard Drozdz
Jagiellonian University, Poland
Background: Carbohydrate metabolism is one of the key metabolic pathways subject to changes during Streptococcus mutans-mixed
biofilm development.
Aim: The objective of this study was determination of the role of GCK and PK in S. mutans pathogenicity.
Material & Methods: Pyruvate kinase and glucokinase from S. mutans-mixed biofilm species were purified, precipitated and
estimated fluorimetrically. The study was performed on type and clinical strains. In total, 21 children with caries were enrolled, (4±1.7
years). As many as 22 individuals without caries (4.12±1.22 years) served as the control group. Phenotyping of isolated bacterial
strains was performed, and evaluated by 16S rDNA gene sequencing. Biofilm assay was carried out according to current protocols in
microbiology.
Results: Out of 100 isolated strains, 74 were classified as S. mutans species. PK and GCK activities were highest after 6 and 12 hours
incubation in the mixed biofilm species. PK activity was higher (1.45 mU/mg of protein) in the experimental group compared to the
control (1.10 mU/mg of protein).
Conclusions: The glycolytic activity increases in the newly formed biofilm after 6 and 12 hours of incubation; however, this activity
decreases with dental plaque biofilm aging. It was demonstrated that the amount of synthesized PK in S. mutans-mixed biofilm
species grows in the caries group. Inhibition of glycolysis metabolic pathway proteins during mixed-species biofilm of S. mutans
development may have an effect on reduction of the development of dental caries in children.
Biography
Wirginia Krzysciak has completed her PhD from Jagiellonian University, Poland. She is an Assistant Professor in the Department of Medical Diagnostics, Faculty of
Pharmacy, Medical College, Jagiellonian University in Krakow, Poland. She has published more than 20 papers in peer-review journals on Caries Pathogenesis and Redox
Signaling. She is a Lecturer and one of the Instructors of Laboratory Medicine where she teaches Hematology, Laboratory Medicine and Medical Diagnostics. She is also a
Founder and the Instructor of Students Association of Laboratory Diagnosticians. She is a Member of Polish Society of Microbiologists and Polish Society of Biochemistry.
Wirginia Krzysciak et al., Biochem Anal Biochem 2016, 5:3(Suppl)