biochemistry-2016_posters-accepted-abstracts

Volume 5, Issue 3(Suppl)

Biochem Anal Biochem 2016

ISSN: 2161-1009, Biochem an open access journal

Page 57

Biochemistry 2016

October 10-12, 2016

conferenceseries

.com

Biochemistry

October 10-12, 2016 Kuala Lumpur, Malaysia

International Conference on

In vivo

anti-hyperlipidemic activity of

Tetracarpidium conophorum

(African walnut) oil

Oriakhi Kelly and Uadia P O

University of Benin, Nigeria

yperlipidemia, a disorder of lipid metabolism characterized by elevated levels of lipids circulating in the blood, has now become

a global concern. It is considered as one of the five leading cause of death in the world. Its prevalence is greatly influenced by

adaptation to sedentary lifestyle and an increase in the consumption of a high fat diet. Hyperlipidemia is strongly linked to the

development of cardiovascular events and metabolic syndrome diseases. Thus, regulating blood lipids levels is vital in the prevention

and treatment of hyperlipidemia and its related diseases. A total of 35 rats were used in this study. The animals were randomly assigned

into seven groups (five rats per group). Group I (control group) was fed with normal diet (ND) only, group II, V, VI and VII were fed

with high cholesterol diet, which contain 1% cholesterol and 0.5% bile salt for five weeks (37 days) to establish hypercholesterolemia,

while groups III and IV were fed with normal diet for five weeks and thereafter administered with 250 and 500 mg/kg body weight of

Tetracarpidium conophorum

oil (TCO) respectively for a period of 20 days. Group II were maintained on hyper cholesterol diet, while

Group V and VI was administered 250 and 500 mg/kg body weight of TCO respectively for a period of 20 days, while group VII was

given 80 mg/kg body weight of atorvastatin used as a reference drug. After six weeks of feeding with the respective diets, rats were

deprived of food overnight. Blood sample was collected and biochemically analyzed for Total Cholesterol (TC), Triglyceride (TG),

High Density Lipoprotein Cholesterol (HDL-C) and Low Density Lipoprotein (LDL-C), Malondialdehyde levels (MDA), Aspartate

Transaminase (AST), Creatine Kinase (CK) and Lactate dehydrogenase (LDH) activities. The results showed that there was significant

increase (P<0.05) in TC, LDL-C, CK, LDH and MDA levels with a reduction in HDL-C in rats induced with high cholesterol diet after

37 days when compared to the initial values at day 0. Oral administration of

Tetracarpidium conophorum

oil and atorvastatin drug

for a period of 20 days resulted in significant lowering (P<0.05) of the levels of TC, LDL-C, CK, LDH and MDA levels with increase

in HDL-C in rats induced with high cholesterol diet. However, there were also significant decrease (P<0.05) in TC, LDL-C, LDH, CK

and MDA levels with increase in HDL-C in rats administered with 250 and 500 mg/kg body weight of

Tetracarpidium conophorum

oil alone for 20 days in rats fed with normal diet when compared to control. There was no statistically significant difference in AST

level in both rats fed with normal and hyper cholesterol diets when compared to control throughout the period of the experiment.

Tetracarpidium conophorum

oil could effectively reduce or control the amount of serum cholesterol and LDL-C. It is apparent that the

oil could contribute to new formulation with significant hypolipidemic effect and cardioprotective properties.

Biochem Anal Biochem 2016, 5:3(Suppl)