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.com
Volume 6, Issue 8 (Suppl)
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13
th
Biotechnology Congress
Abril Ivett Priscilla Gomez-Guzman et al., J Biotechnol Biomater 2016, 6:8(Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.068Evaluation of nutrient removal (NO
3
-N, NH
3
-N and PO
4
-P) with
Chlorella vulgaris
,
Pseudomonas putida,
Bacillus cereus
and the consortium of these microorganisms in the secondary treatment of municipal
wastewater effluents
Abril Ivett Priscilla Gomez-Guzman, Sergio Jimenez-Magana, Cesar Gomez-Hermosillo, Victor Perez-Luna, F Javier Parra-Rodriguez, A Suggey Guerra-Renteria,
Blanca Rosa Aguilar-Uscanga, Josue Solis-Pacheco
and
Orfil Gonzalez-Reynoso
University of Guadalajara, Mexico
M
icroalgae and some bacteria offer a promising technology for the removal of nutrients such as Nitrogen and Phosphorus
in municipal wastewater. In this investigation the microalgae
Chlorella vulgaris
, the bacteria
Pseudomonas putida, Bacillus
cereus
and the consortium microalga/bacteria of these microorganism were used as model in the removal of nutrients using a model
wastewater. The results of removal of nutrients with
Chlorella vulgaris
was 24%, 80.6% and 4.30% for NO
3
-N, NH
3
-N and PO
4
-P,
respectively. For
Bacillus cereus
the removal of nutrients was 8.4%, 28.8% and 3.8% of NO3-N, NH3-N and PO4-P. With
Pseudomonas
putida
was 5%, 41.8% and 4.3% of NO
3
-N, NH
3
-N and PO
4
-P (in 168 hours respectively). The consortium of
Chlorella vulgaris
,
Bacillus cereus, Pseudomonas putida
were able to remove the 29.4%, 4.2% and 0% of NO
3
-N, NH
3
-N and PO
4
-P in 240 hours. The
highest biomass production was found with
Bacillus cereus
450 mg/L followed by
Pseudomonas putida
444 mg/L, the consortium
205 mg/L and
Chlorella vulgaris
88.9 mg/L. This study showed that
Chlorella vulgaris
consume first NH3-N as a source of Nitrogen
and when it is exhausted then consume NO
3
-N, it happen when both compounds are available in the medium. In the removal of
phosphate (PO
4
-P) the microorganisms
Chlorella vulgaris
and
Bacillus cereus
were able to remove a higher amount because they can
assimilate this nutrient even in stress conditions (like changes in pH) such as happening in the municipal wastewater.
Biography
Abril Ivett Priscilla Gomez-Guzman is currently a graduate student in the University of Guadalajara, Jalisco, Mexico. She is pursuing her PhD studies in the program
of Science of Biotechnological Processes. She has completed her Masters studies from the Autonomous University of Baja California and her Bachelor’s degree
studies from the Technological Institute of Tepic in Biochemical Engineering. She was a Professor of the Autonomous University of Guadalajara from 2011-2013.
She has published one paper in reputed journals.
abrilgomezguzman@hotmail.com