9 / 25
Page 61
Notes:
conferenceseries
.com
Volume 8, Issue 3(Suppl)
J Allergy Ther, an open access journal
ISSN: 2155-6121
Allergy-Clinical Immunology 2017
September 07-08, 2017
September 07-08, 2017 | Edinburgh, Scotland
ALLERGY, ASTHMA & CLINICAL IMMUNOLOGY
11
th
International Conference on
Male-dominant suppressive activity of CD8+ T cells on CD4+ T cells: Assessing female-dominant
allergic airway inflammation
Tomomitsu Miyasaka
1
, Chiaki Masuda
1
, Toshiaki Kikuchi
2
, Tasuku Kawano
1
, Motoaki Takayanagi
1
and
Isao Ohno
1
1
Tohoku Medical and Pharmaceutical University, Japan
2
Niigata University, Japan
Statement of the Problem:
Bronchial asthma is more severe in females than in males after puberty because of stronger Th2-
oriented immune response in females. However, the mechanism of the sex difference in asthmatic immune response remains
unclear. CD8+ T cells play an important role in regulating the asthma immune response through their suppressive effect on
Th2 polarization within the localized lymph nodes.
Theoretical Orientation:
In the present study, we investigated the sex-specific effect of CD8+ T cells on the female-predominant
asthmatic immune responses using a mouse model.
Results:
The number of eosinophil in bronchoalveolar lavage (BAL) fluid, lung Th2 cytokine levels, and IL-4 production by
bronchial lymph node cells were significantly higher in wild-type female compared with male mice, whereas no such sex
differences were observed between cd8α-disrupted (CD8KO) male and female mice. The adaptive transfer of wild-type male,
but not female, CD8+ T cells reduced the number of inflammatory cells in the recovered BAL fluid of CD8KO male, but not
female, recipient mice. Male CD8+ T cells produced higher levels of IFN-γ than female CD8+ T cells. Treatment with anti-
IFN-γ antibody completely abrogated the sex difference in the suppressive activity of CD8+ T cells on IL-4 production from
CD4+ T cells. However, IFN-γ receptor expression on CD4+ T cells was higher in male mice than in female mice. rIFN-γ
treatment increased the proportion of IFN-γ receptor α+ CD4+ T cells in male naïve CD4+ T cells more than in female naïve
CD4+ T cells.
Conclusion & Significance:
These results suggest that female-dominant asthmatic immune responses are induced by the
reduced production of IFN-γ by CD8+ T cells and the lower expression of IFN-γ receptor on CD4+ T cells caused by exposure
to IFN-γ in females compared with males.
Biography
Tomomitsu Miyasaka completed his PhD at Tohoku University, Japan. He is an Assistant Professor at Tohoku Medical and Pharmaceutical University, Japan. His
research area is Allergy, and he is interested in determining the mechanism(s) that are responsible for the altered asthma severity by sex or related to psychological
stress.
t-miya13@tohoku-mpu.ac.jpTomomitsu Miyasaka et al., J Allergy Ther 2017, 8:3(Suppl)
DOI: 10.4172/2155-6121-C1-006