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Volume 8, Issue 3(Suppl) J Allergy Ther, an open access journal
ISSN: 2155-6121 Allergy-Clinical Immunology 2017
September 07-08, 2017
September 07-08, 2017 | Edinburgh, Scotland
ALLERGY, ASTHMA & CLINICAL IMMUNOLOGY
11th International Conference on
The increased susceptibility to adult-onset asthma through the inhibition of the development of
respiratory tolerance by early life stress
Tasuku Kawano, Ryusuke Ouchi, Tomomitsu Miyasaka, Yuichi Ohkawara, Tomoko Takahashi, Motoaki Takayanagi and Isao Ohno
Tohoku Medical and Pharmaceutical University, Japan
Statement of the Problem: Allergic asthma is characterized by Th2 type inflammation, essentially due to a breakdown of
immune tolerance to an environmental allergen. Etiologically, experiences of early-life stress have been demonstrated to be
associated with heightened prevalence of adult asthma. However, mechanisms underlying the stress leading to the development
of asthma are poorly understood. Therefore, we examine if early-life stress increases the susceptibility to adult-onset asthma
through the inhibition of the development of the respiratory tolerance.
Methodology & Theoretical Orientation: Female BALB/c pups were sensitized by intraperitoneal injections of ovalbumin
(OVA)/Al(OH)3 on postnatal days (PND) 24 and 29. Respiratory tolerance was induced by the inhalation of OVA on PNDs
18 and 21 before the sensitization. Maternal separation (MS) was used as a model of early-life stress and repeated from PND
17 to 22. On PND 76, the mice were challenged with OVA aerosol. Airway inflammation was evaluated with numbers of
inflammatory cells in bronchoalveolar lavage fluid (BALF). The contents of IFN-Υ, IL-4, IL-5 and IL-13 in BALF were measured
by ELISA. The airway hyper-responsiveness (AHR) was assessed by methacholine-induced airflow obstruction.
Conclusion & Significance: In tolerized mice, the numbers of inflammatory cells, the cytokine contents and AHR were
remarkably decreased compared with those in non-tolerized mice. However, these effects of the tolerance were significantly
reduced by MS exposure. These results suggested that early-life stress exposure has a potential to increase the risk of adult-
onset asthma through the inhibition of the development of immune tolerance.
Biography
Tasuku Kawano completed his PhD at Tohoku Medical and Pharmaceutical University in 2009. He is presently working as an Assistant Professor in the Department
of Pathophysiology at Tohoku Medical and Pharmaceutical University. He aims to reduce asthma patients. He studies elucidation of asthmatic onset mechanism
based on nerve-endocrine-immunity axis. He has been trying for the creation of new fields for the development of novel medicines and preventive drugs in
asthma. He has published a paper, “The involvement of central nervous system histamine receptors in psychological stress-induced exacerbation of allergic airway
inflammation in mice” in Allergol Int. 2016 Sep; 65 Suppl:S38-44.
Tasuku Kawano et al., J Allergy Ther 2017, 8:3(Suppl)
DOI: 10.4172/2155-6121-C1-006