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Volume 07 Advances in Crop Science and Technology
ISSN: 2329-8863 Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14th International Conference on
Agriculture & Horticulture
QTL mapping of nitrogen deficiency tolerance by genome-wide association study approach in a nested
association mapping (NAM) population of rice
Kai Chen, Jianlong Xu and Zhikang Li
Chinese Academy of Agricultural Sciences, China
To develop green super rice varieties with high yield and improved nitrogen deficiency tolerance (NDT), a
nested association mapping population was developed through a modified backcross breeding approach using
a high yielding and widely adaptable Xian variety, Huanghuazhan (HHZ) as the recipient and 8 donor parents
IR50, IR64, Teqing, PSBRC28, PSBRC66, CDR22, OM1723 and Phalguna. A total of 496 lines, plus the HHZ, were
used for evaluation of heading date, plant height, grain yield, biomass yield, thousand grain weight under the low
nitrogen (LN) and the normal nitrogen (NN) conditions in 2013 early season, 2013 last season and 2014 early
season. Total of 48 QTLs were identified by R-package MAGICqtl based on 7388 bins derived from 400K high-
quality SNPs under the LN, NN and LN/NN conditions. Among them, ten main-effect QTLs were simultaneously
identified in the LN and NN conditions. Four genomic regions, including bin16 on chromosome 1, bin 2186 on
chromosome 3, bin 3699 on chromosome 6, and bin 4859 on chromosome 8 were simultaneously identified for
NDT-related traits. The qTGW2-1 for thousand grain weight, which was simultaneously detected across three
seasons under the LN condition, was delimited into a region of 50Kb by genotypic overlapping of recombinant
lines inside the marker bin1459. The donor alleles at qTGW2-1 contribute to NDT. Molecular designed pyramiding
of favorable alleles at the consistently detected NDT-QTLs is under way to develop new breeding lines with high
yield and NDT based on performance of NDT and QTL information of NAM lines in the elite background.
Recent Publications
1. Yu J, Holland JB, McMullenMD, et al (2008). Genetic design and statistical power of nested association mapping
in maize. Genetics 178: 539–551
2. Buckler ES, Holland JB, Bradbury PJ, et al. (2009). The genetic architecture of maize. Science 325: 714–718.
3. Hu B, Wang W, Ou SJ, et al (2015) Variation in NRT1.1B contributes to nitrate-use divergence between rice
subspecies. Nature Genetics, 47(7): 834-838
4. Sun HY, Qian Q, Wu K, et al (2014) Heterotrimeric G proteins regulate nitrogen-use efficiency in rice. nature
genetics, 46: 652-657.
5. Bo Feng, Kai Chen, Yanru Cui, et al (2018) Genetic dissection and simultaneous improvement of drought and
low nitrogen tolerances by designed QTL pyramiding in rice. Frontiers in Plant Science, 2018.9:306
Biography
Kai Chen got PhD degree on Crop Sciences in 2012 in Shenyang Agricultural University, China. After finishing postdoctoral career of molecular rice breeding
for four years at CAAS, he joined the Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, focusing on molecular breeding
for hybrid rice and inbred rice for high yield, high grain quality, abiotic and biotic stress tolerances. So far, he has published 5 papers in international journals.
Kai Chen et al., Adv Crop Sci Tech 2019, Volume 07