4 / 45
Page 70
conferenceseries
.com
Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
A non-chemical approach for controlling Panama disease occurrence in Taiwan with the essential oil of
Biden pilosa
Zhong-Bin Wu
1
, Ying-Hong Lin
2
, Anren Hu
3
, Fen-Lien Chi
1
and
Jyh-Shyan Tsay
1
1
National Taitung Jr. College, Taiwan
2
National Pingtung University of Science and Technology, Taiwan
3
Tzu Chi University, Taiwan
P
anama disease caused by
Fusarium oxysporum
f. sp.
cubense
(FOC) is the most destructive disease in banana
production. In this study, we explore a potential non-chemical approach to reducing the incidence of Panama
disease in fields in Taiwan. Fresh materials collected from 11 naturalized or invasive plants were subjected to steam-
distillation, and the isolated essential oils or hydrosols were subsequently evaluated for in vitro antifungal activity
against three FOC reference isolates:
YJL-F040
race
1, ATCC-76243
race 2, and
ATCC-38741
subtropical race 4.
The essential oil of the Biden pilosa plant demonstrated potent antifungal activity against FOC isolates, showing
22% to 43% inhibition of mycelial growth in races 1, 2, 4, and 70% inhibition of spore germination in race 4. A
preliminary field experiment was then conducted in an orchard in Taitung County, Taiwan, and the area was divided
into four sections, and one testing area and three control areas were randomly assigned. A re-dissolved 0.01% (v./v.)
solution prepared from the essential oils and hydrosol of
B. pilosa
plants were irrigated into the bulk soil areas of
84 banana plantlets during secondary hardening, once a week beginning March 13, 2017. The incidence of Panama
disease was investigated from August 28 to November 15, 2017. Disease incidence was just 16% in the testing area,
compared to 53%, 51%, and 12% in the three control areas. A second investigation was carried out on four different
orchards from March 20, 2018 to December 21, 2018. Incidence rates of Panama disease were 22.2% to 27.3% in the
testing areas and 45.2% to 62.2% in the control areas. In-depth analysis of the antifungal compounds in
B. pilosa
essential oils was carried out with gas chromatography–mass spectrometry. Our results suggest a promising non-
chemical approach for controlling the occurrence of Panama disease using
B. pilosa
essential oils.
Zhong-Bin Wu et al., Adv Crop Sci Tech 2019, Volume 07