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Volume 07 Advances in Crop Science and Technology
ISSN: 2329-8863 Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14th International Conference on
Agriculture & Horticulture
Effect of Symphytum Officinale, Cymbopogon Citratus and Allium sativum extracts on Botrytis Cinerea
causal agent of grey mold of tomato
Louanchi Meriem, Bouzit Feriel and Hammad Massinissa
Ecole Nationale Supérieure Agronomique (ENSA), Algeria
B otrytis cinerea Pers. is a necrotrophic plant pathogenic fungus, the causal agent of gray mold. The fungus has a wide
host range and can infect over 230 plant species. In Algeria, it is almost present on all greenhouse crops, especially
tomatoes; which the pathogen can cause major economic damages. This disease is controlled mainly by the application of
fungicides. However, this can lead to the development of pathogen resistance. For this reason, some alternative methods of
control have been adopted and our research is focused on them by using plant extracts against B. cinerea. In this study, we
evaluated the effect of comfrey manure (Symphytum officinale), decoction of citronella ( Cymbopogon citratus) and garlic
decoction (Allium sativum) on one strain of Botrytis cinearea isolated from tomato and selected among 40 isolates for its
high aggressivity and resistance to fenhexamid. Tests were conducted in vitro, on PDA medium for mycelial growth and
on deatached leaves by measuring the diameter of lesion. Different concentrations of plant extracts (5-10 and 20%) were
tested on PDA medium. The results revealed that the highest antimicrobial activity (50% inhibition of mycelial growth)
was obtained using Symphytum officinale at 10%. On detached leaves, the concentration of 5% was tested for all extracts,
before and after inoculations. Results showed that Symphytum officinale reduce the severity of the disease by 52% in
preventive and 44% in curative treatment. Whereas, Allium sativum revealed the highest efficacy on reducing the severity
of the disease by 76% in preventive and 64% in curative treatment.
Adv Crop Sci Tech 2019, Volume 07