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Volume 6, Issue 4 (Suppl) Agrotechnology, an open access journal
ISSN: 2168-9881 Agri 2017
October 02-04, 2017
allied
academies
10th International Conference on
AGRICULTURE & HORTICULTURE
October 02-04, 2017 London, UK
Identification and diversity analysis of the four-major host-defense peptide families in quail
Kei Hanzawa
Tokyo University of Agriculture, Japan
Statement of the Problem: Quails are bred for their meat and eggs in East Asia, and are widely used as experimental model
animals at research institutes around the world. We are currently focusing on subclinical infection in quail with infectious
diseases, such as influenza. Utilization of innate immunity has the potential to reduce the use of antibiotics and to control the
emergence of drug-resistant bacteria. We identified the gene families of four quail host-defense peptides (HDPs): NK-lysin,
liver expressed antimicrobial peptide 2 (LEAP-2), cathelicidin (CATH) and β-defensin (AvBD), and investigated their genetic
diversity.
Materials & Methods: Genome information was obtained from DNA derived from blood samples collected from 99 quails in
6 lines. Nucleotide sequences were determined by capillary sequencing and next-generation sequencing. The obtained base
sequence was analyzed by Genetyx Mac and CLC Genomics Workbench software packages.
Findings: Quail NK-lysin consisted of a single locus, suggesting that amino acid substitution in the antibacterial active region
is closely related to antibacterial activity. LEAP-2 also consisted of a single locus, no polymorphisms were observed in the
amino acid sequence, and the amino acid sequence of mature peptides was consistent with that found in chicken. CATHs
consisted of four loci homologous to those found in chicken, with amino acid substitutions in two loci. AvBDs were basically
composed of 12 loci, 1 to 5 copy number variation (CNV) associated with gene duplication was observed at one locus with
numerous amino acid substitutions.
Conclusion & Significance: The gene structure of major quail HDPs and their DNA polymorphisms were clarified. Future
studies will examine the effects of DNA polymorphisms in HDPs on antibacterial activity in vitro , and clarify the relationship
between HDPs, innate immunity receptors (Toll like receptor: TLR) and enterobacterial flora
Biography
Kei Hanzawa is a Professor in Department of Animal science, Faculty of Agriculture, Tokyo University of Agriculture. He researches animal physiological genetics:
particularly identification and diversity analysis of quail major histocompatibility complex gene region and host defense peptides genes, gene identification and
analysis of heat shock response of quail heat shock protein 70s and 90s, and equine membrane transporters and exercise physiology of erythrocytes. He is the
Vice President of the Japan Society of Animal Science and the Auditor of the Japanese Society of Poultry Science.
Kei Hanzawa, Agrotechnology 2017, 6:4(Suppl)
DOI: 10.4172/2168-9881-C1-028