agri-2017-posters-accepted-abstracts

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Volume 6, Issue 4 (Suppl)

Agrotechnology, an open access journal

ISSN: 2168-9881

Agri 2017

October 02-04, 2017

allied

academies

International Conference on

AGRICULTURE & HORTICULTURE

October 02-04, 2017 London, UK

Notes:

One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection

for barley virus diseases

Bong Choon Lee, Ju Young Bae, Sang Min Kim, Ji-Eun Ra, Nak Jung Choi, Man Young Choi

and

Ki Do Park

National Institute of Crop Science, RDA, South Korea

irus disease surveys on barley cultivation areas were conducted during 2014-2015 in Korea. In this result, we detected

Barley yellow mosaic virus

(BaYMV),

Barley mild mosaic virus

(BaMMV) and

Barley yellow dwarf virus

(BYDV) by RT-

PCR from barley leaves. These viruses are of great economic importance for wheat and barley, causing significant quantitative

and qualitative losses in yield. In this study, a multiplex reverse transcription polymerase chain reaction (mRT-PCR) method

was developed for the simultaneous detection of these three viruses. Virus-infected barley leaves were collected from naturally

infected barley in several provinces of Korea. Primers were designed from conserved regions of the capsid protein (CP) gene of

viruses and the GenBank accession numbers for the CP coding genes are as follows: D01091(BaYMV), L49381(BaMMV), and

NC021481(BYDV). Three sets of specific primers targeted to the CP coding genes of BaYMV, BaMMV and BYDV were used

to amplify fragments that were 594 bp, 461 bp, and 290 bp, respectively. The one-step mRT-PCR assay proved to be a rapid and

specific barley virus diagnostic tool. The diagnosis of mixed infections with barley viruses in one single reaction will reduce

the cost, significantly increase the number of samples that can be analyzed, which will allow an examination of the viral disease

epidemiology in barley.

Biography

Bong Choon Lee has completed from Hokkaido University in Japan and Postdoctoral studies from Chonnam National University in Korea. She is the Senior

Researcher of National Institute of Crop Science, RDA, Korea. She has published more than 50 papers in journals and has been serving as an Editorial Board

Member of Research in

Plant Disease Journal

in Korea.

leebc21@korea.kr

Bong Choon Lee et al., Agrotechnology 2017, 6:4(Suppl)

DOI: 10.4172/2168-9881-C1-028