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Volume 6, Issue 4 (Suppl) Agrotechnology, an open access journal
ISSN: 2168-9881 Agri 2017
October 02-04, 2017
allied
academies
10th International Conference on
AGRICULTURE & HORTICULTURE
October 02-04, 2017 London, UK
Pantoea ananatis causing grain discoloration is widely distributed in the rice-growing fields of the
Republic of Korea
Kim Sang -Min, Ra Ji-Eun, Seo Su Jwa, Lee Bong Choon, and Choi Nak Jung
National Institute of Crop Science, Republic of Korea
We collected local isolates of Pantoea ananatis causing bacterial grain discoloration from southern rice-growing fields
in Korea and constructed phylogenetic tree to see the genetic variation of P. ananatis. In 2016, rice panicles showing
symptoms of grain discoloration and grain abortion were collected and the inside of lemma and palea were streaked on the
King’s B agar plate for pathogen isolation. 63 regional isolates were randomly selected for avoiding selection bias and gDNA
were extracted for gene-based species identification. Based on 16S rRNA gene, B. glumae, the major pathogen of grain rot,
amounted for 49.2% and Pantoea spp. amounted for 22.2%, separately. Multilocus sequence analysis (MLSA) by four gyrB,
rpoB, inf B, and atpD identified Pantoea spp. as P. ananatis. The pathogenicity of collected P. ananatis isolators were tested and
showed weak symptoms of stem rot by artificial injection and weak symptoms of grain discoloration by spraying to the panicles
of rice. In this study, we show the incidence and the regional distribution of P. ananatis, and the phylogenetic relationship
among P. ananatis regional isolates in Korea, 2016. As the risk of the grain rot and grain discoloration is increasing according
to the climatic change, it would be the basic data for understanding P. ananatis in the Republic of Korea.
Biography
He is a researcher for plant pathology in the National Institute of Crop Science, RDA. Main works include diagnosis, identification and control of crop diseases on
the field and on the lab.
Kim Sang -Min et al., Agrotechnology 2017, 6:4(Suppl)
DOI: 10.4172/2168-9881-C1-028