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Volume 8 Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4th International Conference on
Advances in Biotechnology and Bioscience
Partially reduced graphene oxide as a fluorescent probe and its interaction with liver cancer cells:
DUV imaging study
Vladimir Djokovic
University of Belgrade, Serbia
Partially reduced graphene oxide (prGO), an intermediate form between graphene oxide (GO) and graphene, was studied
as a potential probe for fluorescent bioimaging of cancer cells. prGO material was obtained by the reduction of the initially
prepared graphene oxide nano-sheets with hydrazine. The fluorescence of the GO sheets increases with time of the reduction
due to change in ratio of the sp2 and sp3 carbon sites. It was found that the fluorescence intensity reached its maximum
after reduction for six minutes and the prGO sample was extracted from the dispersion at this particular time. After that,
the reduction process was left to proceed further to saturation until highly reduced graphene oxide (rGO) was obtained.
Scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS) was used to study
the morphology and structure of the GO, prGO and rGO samples. Their optical properties were investigated by UV-vis and
photoluminescence (PL) spectroscopies. The fluorescent GO, prGO and rGO nanosheets were used in the DUV fluorescence
imaging studies of the cancer liver cell line Huh7.5.1, which were performed on DISCO beamline of synchrotron SOLEIL.
The internalization of prGO sheets by the cells resulted in a strong increase in the intensity of fluorescence signal, which was
2.5 times higher than the intensity of the autofluorescence of the control sample. Also, time-lapse fluorescence microscopy
experiments showed that the dynamics of the fluorescent signals changes after incubation of the cells with GO, prGO and rGO
sheets. The prGO nanostructure was suggested as a possible carrier for cancer drugs, since it could be easily conjugated with
aromatic ring containing molecules.
Biography
Vladimir Djokovic has completed his PhD in Physics at University Belgrade, Faculty of Physics in 1999. He spent two years as a Postdoctoral Fellow at University of the
Free State, SouthAfrica. In the last couple of years, he was a Visiting Professor/Researcher at NASAUniversity Research Center, North Carolina Central University. So far,
he has published two book chapters and more than 60 papers in ISI journals. He is a Leader of Polymer Nanocomposite group at Vinča Institute from 2005 and a Professor
of Polymer Physics in the Faculty of Physics at University of Belgrade.
Vladimir Djokovic, J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-103